Signaling lymphocytic activation molecule (SLAM)/SLAM-associated protein pathway regulates human B-cell tolerance

• Published in: Journal of allergy and clinical immunology Vol. 133; no. 4; pp. 1149 - 1161
• Main Authors: Menard, Laurence, Cantaert, Tineke, Chamberlain, Nicolas, Tangye, Stuart G., Riminton, Sean, Church, Joseph A., Klion, Amy, Cunningham-Rundles, Charlotte, Nichols, Kim E., Meffre, Eric
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.04.2014; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Antigens; Antigens, CD - metabolism; Autoimmunity - genetics; Autoimmunity - immunology; B-Cell Activating Factor - blood; B-cell tolerance; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression; Humans; Immune system; Immune Tolerance; Immunopathology; Intracellular Signaling Peptides and Proteins - deficiency; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Kinases; Lymphocyte Activation - immunology; Lymphoproliferative Disorders - genetics; Lymphoproliferative Disorders - immunology; Lymphoproliferative Disorders - metabolism; Medical sciences; Mutation; Protein Binding; Protein Transport; Receptors, Antigen, B-Cell - metabolism; Receptors, Cell Surface - metabolism; regulatory T cells; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Signal Transduction; signaling lymphocytic activation molecule; Signaling Lymphocytic Activation Molecule Associated Protein; Signaling Lymphocytic Activation Molecule Family; Signaling Lymphocytic Activation Molecule Family Member 1; SLAM-associated protein; T cell receptors; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - metabolism; BAFF; TCR; SAP; SLAM; Treg; B-cell tolerance; CFSE; FOXP3; signaling lymphocytic activation molecule; regulatory T cells; BCR; SLAM-associated protein; Tresp; ANA; APC; PE; NKT; IPEX; SHP; TLR; XLP; B cell–activating factor of the TNF family; Anti-nuclear antibody; Natural killer T; B-cell receptor; T-cell receptor; Forkhead box protein 3; X-linked lymphoproliferative disease; Allophycocyanin; Regulatory T; Src homology domain 2–containing protein tyrosine phosphatase; Toll-like receptor; Responder T; Immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Carboxyfluorescein succinimidyl ester; Phycoerythrin; Human; Immunopathology; Tolerance; B-Lymphocyte; Regulation(control); Immunology; T-Lymphocyte; Signaling lymphocyte activation molecule; Regulatory cell; SLAM associated protein
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2013.10.051

Signaling lymphocytic activation molecule (SLAM)–associated protein (SAP) can mediate the function of SLAM molecules, which have been proposed to be involved in the development of autoimmunity in mice. We sought to determine whether the SLAM/SAP pathway regulates the establishment of human B-cell tolerance and what mechanisms of B-cell tolerance could be affected by SAP deficiency. We tested the reactivity of antibodies isolated from single B cells from SAP-deficient patients with X-linked lymphoproliferative disease (XLP). The expressions of SAP and SLAM family members were assessed in human bone marrow–developing B cells. We also analyzed regulatory T (Treg) cell function in patients with XLP and healthy control subjects. We found that new emigrant/transitional B cells from patients with XLP were enriched in autoreactive clones, revealing a defective central B-cell tolerance checkpoint in the absence of functional SAP. In agreement with a B cell–intrinsic regulation of central tolerance, we identified SAP expression in a discrete subset of bone marrow immature B cells. SAP colocalized with SLAMF6 only in association with clustered B-cell receptors likely recognizing self-antigens, suggesting that SLAM/SAP regulate B-cell receptor–mediated central tolerance. In addition, patients with XLP displayed defective peripheral B-cell tolerance, which is normally controlled by Treg cells. Treg cells in patients with XLP seem functional, but SAP-deficient T cells were resistant to Treg cell–mediated suppression. Indeed, SAP-deficient T cells were hyperresponsive to T-cell receptor stimulation, which resulted in increased secretion of IL-2, IFN-γ, and TNF-α. SAP expression is required for the counterselection of developing autoreactive B cells and prevents their T cell–dependent accumulation in the periphery.