First isolation of Leishmania from Northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex

Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes w...

Full description

Saved in:
Bibliographic Details
Published inPLoS neglected tropical diseases Vol. 8; no. 12; p. e3339
Main Authors Pothirat, Thatawan, Tantiworawit, Adisak, Chaiwarith, Romanee, Jariyapan, Narissara, Wannasan, Anchalee, Siriyasatien, Padet, Supparatpinyo, Khuanchai, Bates, Michelle D, Kwakye-Nuako, Godwin, Bates, Paul A
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 01.12.2014
Public Library of Science (PLoS)
Subjects
Biology and Life Sciences
Care and treatment
Case studies
Deoxyribonucleic acid
Diagnosis
DNA
Genetic aspects
Humans
Identification and classification
Leishmania
Leishmania enriettii
Leishmania enriettii - classification
Leishmania enriettii - genetics
Leishmania enriettii - isolation & purification
Leishmaniasis
Leishmaniasis, Visceral - drug therapy
Leishmaniasis, Visceral - parasitology
Male
Medicine
Medicine and Health Sciences
Middle Aged
Parasites
Parasitic diseases
Phylogenetics
Phylogeny
Protozoa
RNA polymerase
Sequence Analysis, DNA
Thailand
Thailand
Online AccessGet full text

Cover

More Information
Summary:Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as "L. siamensis" also appear to be L. martiniquensis.
Bibliography:ObjectType-Case Study-2
SourceType-Scholarly Journals-1
ObjectType-Feature-4
content type line 23
ObjectType-Report-1
ObjectType-Article-3
ObjectType-Article-1
ObjectType-Feature-2
Conceived and designed the experiments: RC NJ PAB. Performed the experiments: TP AT NJ AW PS MDB GKN. Analyzed the data: AT RC NJ KS PAB. Wrote the paper: TP RC NJ KS PAB.
The authors have declared that no competing interests exist.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0003339