Major Persistent 5' Terminally Deleted Coxsackievirus B3 Populations in Human Endomyocardial Tissues

• Published in: Emerging infectious diseases Vol. 22; no. 8; pp. 1488 - 1490
• Main Authors: Bouin, Alexis, Nguyen, Yohan, Wehbe, Michel, Renois, Fanny, Fornes, Paul, Bani-Sadr, Firouze, Metz, Damien, Andreoletti, Laurent
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01.08.2016; Centers for Disease Control and Prevention
• Subjects: 5′ terminally deleted populations; coxsackievirus B3; Coxsackievirus infections; Coxsackievirus Infections - pathology; Coxsackievirus Infections - virology; Coxsackieviruses; Diagnosis; Dispatch; DNA Mutational Analysis; DNA sequencing; Endocarditis - virology; endomyocardial; Enterovirus B, Human - genetics; Female; Genetic aspects; heart; Heart - virology; Humans; idiopathic dilated cardiomyopathy; Life Sciences; Middle Aged; Myocardium - pathology; Nucleotide sequencing; Physiological aspects; RNA, Viral; Sequence Deletion; viruses; France; coxsackievirus B3; viruses; 5′ terminally deleted populations; enterovirus; endomyocardial; idiopathic dilated cardiomyopathy; human; heart
• ISSN: 1080-6040; 1080-6059
• DOI: 10.3201/eid2208.160186

We performed deep sequencing analysis of the enterovirus 5' noncoding region in cardiac biopsies from a patient with dilated cardiomyopathy. Results displayed a mix of deleted and full-length coxsackievirus B3, characterized by a low viral RNA load (8.10(2) copies/μg of nucleic acids) and a low viral RNA positive-sense to RNA negative-sense ratio of 4.8.