Genome-wide identification and expression analysis of HSP90 gene family in Nicotiana tabacum

• Published in: BMC genetics Vol. 20; no. 1; p. 35
• Main Authors: Song, Zhaopeng, Pan, Feilong, Yang, Chao, Jia, Hongfang, Jiang, Houlong, He, Fan, Li, Najia, Lu, Xiaochong, Zhang, Hongying
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.03.2019; BioMed Central; BMC
• Subjects: Abiotic stress; Abscisic acid; Acids; Adenosine triphosphatase; Amino Acid Motifs; ATPases; Bioinformatics; Cellular stress response; Chaperones; Conserved Sequence; Cytoplasm; Drought; Evolutionary genetics; Exons - genetics; Expression pattern; Gene duplication; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genetic aspects; Genome, Plant - genetics; Genome-wide analysis; Genome-wide association studies; Genomes; Genomics; Heat; Heat shock protein 90; Heat shock proteins; Heat tolerance; HSP90 Heat-Shock Proteins - chemistry; HSP90 Heat-Shock Proteins - genetics; Hsp90 protein; Identification; Kinases; Nicotiana - genetics; Nicotiana - physiology; Nicotiana tabacum; Osmotic stress; Peptides; Phylogenetic analysis; Phylogenetics; Phylogeny; Proteins; Sequence Alignment; Species; Stress; Stress, Physiological - genetics; Tobacco; Tobacco (Plant); Transcription factors; Phylogenetic analysis; Nicotiana tabacum; Expression pattern; Genome-wide analysis; Heat shock protein 90
• ISSN: 1471-2156; 1471-2156
• DOI: 10.1186/s12863-019-0738-8

Heat shock proteins 90 (HSP90s) are a highly conserved protein family of cellular chaperones widely found in plants; they play a fundamental role in response to biotic and abiotic stresses. The genome-wide analysis of HSP90 gene family has been completed for some species; however, it has been rarely reported for the tobacco HSP90 genes. In this study, we systematically conducted genome-wide identification and expression analysis of the tobacco HSP90 gene family, including gene structures, evolutionary relationships, chromosomal locations, conserved domains, and expression patterns. Twenty-one NtHSP90s were identified and classified into eleven categories (NtHSP90-1 to NtHSP90-11) based on phylogenetic analysis. The conserved structures and motifs of NtHSP90 proteins in the same subfamily were highly consistent. Most NtHSP90 proteins contained the ATPase domain, which was closely related to conserved motif 2. Motif 5 was a low complexity sequence and had the function of signal peptide. At least 6 pairs of NtHSP90 genes underwent gene duplication, which arose from segment duplication and tandem duplication events. Phylogenetic analysis showed that most species expanded according to their own species-specific approach during the evolution of HSP90s. Dynamic expression analysis indicated that some NtHSP90 genes may play fundamental roles in regulation of abiotic stress response. The expression of NtHSP90-4, NtHSP90-5, and NtHSP90-9 were up-regulated, while NtHSP90-6, and NtHSP90-7 were not induced by ABA, drought, salt, cold and heat stresses. Among the five treatments, NtHSP90s were most strongly induced by heat stress, and weakly activated by ABA treatment. There was a similar response pattern of NtHSP90s under osmotic stress, or extreme temperature stress. This is the first genome-wide analysis of Hsp90 in N. tabacum. These results indicate that each NtHSP90 member fulfilled distinct functions in response to various abiotic stresses.