The effect of protozoa on the composition of rumen bacteria in cattle using 16S rRNA gene clone libraries

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 3; pp. 499 - 506
• Main Authors: Ozutsumi, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu (Japan)), Tajima, K, Takenaka, A, Itabashi, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.03.2005; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 16S rRNA gene; Animals; Bacteria - isolation & purification; Base Sequence; Biological and medical sciences; CATTLE; CLONES; Cloning, Molecular; DNA Primers; Eukaryota - physiology; FAUNA; Fundamental and applied biological sciences. Psychology; LIBRARIES; phylogenetic analysis; PHYLOGENY; Polymerase Chain Reaction; PROTOZOA; RNA; RNA, Ribosomal, 16S - genetics; rumen; Rumen - microbiology; Rumen - parasitology; RUMEN MICROORGANISMS; TAXONOMY; Protozoa; phylogenetic analysis; Ribosomal DNA; 16S-RNA; Bacteria; Rumen; Livestock; cattle; Gene library; Phylogeny; 16S rRNA gene

The effect of the presence of protozoa on the composition of rumen bacteria was investigated in cattle. Seven castrated Holstein cattle were divided into two groups: four faunated and three unfaunated, and 16S ribosomal RNA gene (rDNA) clonal libraries were constructed. A total of 312 clones were se...