The effect of protozoa on the composition of rumen bacteria in cattle using 16S rRNA gene clone libraries

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 3; pp. 499 - 506
• Main Authors: Ozutsumi, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu (Japan)), Tajima, K, Takenaka, A, Itabashi, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.03.2005; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 16S rRNA gene; Animals; Bacteria - isolation & purification; Base Sequence; Biological and medical sciences; CATTLE; CLONES; Cloning, Molecular; DNA Primers; Eukaryota - physiology; FAUNA; Fundamental and applied biological sciences. Psychology; LIBRARIES; phylogenetic analysis; PHYLOGENY; Polymerase Chain Reaction; PROTOZOA; RNA; RNA, Ribosomal, 16S - genetics; rumen; Rumen - microbiology; Rumen - parasitology; RUMEN MICROORGANISMS; TAXONOMY; Protozoa; phylogenetic analysis; Ribosomal DNA; 16S-RNA; Bacteria; Rumen; Livestock; cattle; Gene library; Phylogeny; 16S rRNA gene
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.69.499

The effect of the presence of protozoa on the composition of rumen bacteria was investigated in cattle. Seven castrated Holstein cattle were divided into two groups: four faunated and three unfaunated, and 16S ribosomal RNA gene (rDNA) clonal libraries were constructed. A total of 312 clones were sequenced across 1.500 bp. The 151 sequences of the faunated cattle were classified into 98 operational taxonomic units (OTUs) having at least 97% similarity. The sequences derived from the faunated cattle were classified into Firmicutes (59.7%), Bacteroidetes (34.4%), Spirochaetes (2.6%), Actinobacteria (2.0%), and Proteobacteria (1.3%). Bacteroides and Prevotella (34.4%) were the major groups in the faunated cattle. The 161 sequences in the unfaunated cattle were classified into 72 OTUs. The sequences derived from the unfaunated libraries were classified into Firmicutes (65.7%), Bacteroidetes (31.1%), Proteobacteria (1.9%), and Spirochaetes (1.2%). The Clostridium botulinum group and its relatives (36.0%) were the major groups in the unfaunated cattle. An analysis by the computer program LIBSHUFF clarified that the presence of ruminal protozoa markedly affected the composition of rumen bacteria.