CD36 regulates substrates utilisation in brown adipose tissue of spontaneously hypertensive rats: In vitro study

• Published in: PloS one Vol. 18; no. 4; p. e0283276
• Main Authors: Silhavy, Jan, Mlejnek, Petr, Šimáková, Miroslava, Marková, Irena, Malínská, Hana, Hüttl, Martina, Kazdová, Ludmila, Kazantsev, Dmitry, Mancini, Massimiliano, Novotný, Jiří, Pravenec, Michal
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.04.2023; Public Library of Science (PLoS)
• Subjects: Adipocytes; Adipose tissue; Adipose tissue (brown); Adipose Tissue, Brown - metabolism; Adipose tissues; Animals; Biology and Life Sciences; Body fat; CD36 antigen; CD36 Antigens - genetics; CD36 Antigens - metabolism; Dextrose; Fatty acids; Fatty Acids - metabolism; Gene expression; Genes; Genetic engineering; Glucose; Glucose - metabolism; Glucose transport; Glycerol; Glycogen; Glycogen synthase kinase 3; Glycogen Synthase Kinase 3 beta - metabolism; Glycogens; Hypertension; Insulin; Intracellular; Kinases; Lipids; Lipogenesis; Medicine and Health Sciences; Oxidation; Palmitates - metabolism; Palmitic acid; Phosphorylation; Physical Sciences; Rats; Rats, Inbred SHR; Rats, Transgenic; Statistical significance; Substrates; Synthesis; Thermogenesis; Translocase; Triglycerides; Type 2 diabetes; Czech Republic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0283276

Thermogenesis in brown adipose tissue (BAT) uses intracellular triglycerides, circulating free fatty acids and glucose as the main substrates. The objective of the current study was to analyse the role of CD36 fatty acid translocase in regulation of glucose and fatty acid utilisation in BAT. BAT isolated from spontaneously hypertensive rat (SHR) with mutant Cd36 gene and SHR-Cd36 transgenic rats with wild type variant was incubated in media containing labeled glucose and palmitate to measure substrate incorporation and oxidation. SHR-Cd36 versus SHR rats showed significantly increased glucose incorporation into intracellular lipids associated with reduced glycogen synthase kinase 3β (GSK-3β) protein expression and phosphorylation and increased oxidation of exogenous palmitate. It can be concluded that CD36 enhances glucose transport for lipogenesis in BAT by suppressing GSK-3β and promotes direct palmitate oxidation.