Probiotic Lactobacillus fermentum TSF331, Lactobacillus reuteri TSR332, and Lactobacillus plantarum TSP05 improved liver function and uric acid management-A pilot study

• Published in: PloS one Vol. 19; no. 7; p. e0307181
• Main Authors: Lin, Jia-Hung, Lin, Chi-Huei, Kuo, Yi-Wei, Liao, Chorng-An, Chen, Jui-Fen, Tsai, Shin-Yu, Li, Ching-Min, Hsu, Yu-Chieh, Huang, Yen-Yu, Hsia, Ko-Chiang, Yeh, Yao-Tsung, Ho, Hsieh-Hsun
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24.07.2024; Public Library of Science (PLoS)
• Subjects: Adult; Alanine; Alanine transaminase; Alanine Transaminase - blood; Aspartate; Aspartate aminotransferase; Aspartate Aminotransferases - blood; Bacteria; Biochemistry; Biology and Life Sciences; Caco-2 Cells; Clinical trials; Dietary supplements; Disturbances; Double-Blind Method; Fatty liver; Fecal microflora; Feces; Feces - microbiology; Female; Functional foods & nutraceuticals; Gastrointestinal Microbiome - drug effects; Genetic testing; Glucose; Hep G2 Cells; Humans; Intestinal microflora; Kinases; Lactobacilli; Lactobacillus fermentum; Lactobacillus plantarum; Lactobacillus plantarum - physiology; Lactobacillus reuteri; Limosilactobacillus fermentum; Limosilactobacillus reuteri; Lipids; Liver; Liver - metabolism; Liver diseases; Male; Medicine and Health Sciences; Metabolic disorders; Metabolites; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Middle Aged; Obesity; Oxidative stress; Pilot Projects; Population studies; Probiotics; Probiotics - administration & dosage; Transaminases; Uric acid; Uric Acid - blood; Uric Acid - metabolism; Canada; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0307181

Metabolic-associated fatty liver disease (MAFLD) is predominantly associated with metabolic disturbances representing aberrant liver function and increased uric acid (UA) levels. Growing evidences have suggested a close relationship between metabolic disturbances and the gut microbiota. A placebo-controlled, double-blinded, randomized clinical trial was therefore conducted to explore the impacts of daily supplements with various combinations of the probiotics, Lactobacillus fermentum TSF331, Lactobacillus reuteri TSR332, and Lactobacillus plantarum TSP05 with a focus on liver function and serum UA levels. Test subjects with abnormal levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and UA were recruited and randomly allocated into six groups. Eighty-two participants successfully completed the 60-day intervention without any dropouts or occurrence of adverse events. The serum AST, ALT, and UA levels were significantly reduced in all treatment groups ( P < 0.05). The fecal microbiota analysis revealed the intervention led to an increase in the population of commensal bacteria and a decrease in pathobiont bacteria, especially Bilophila wadsworthia . The in vitro study indicated the probiotic treatments reduced lipid accumulation and inflammatory factor expressions in HepG2 cells, and also promoted UA excretion in Caco-2 cells. The supplementation of multi-strain probiotics (TSF331, TSR332, and TSP05) together can improve liver function and UA management and may have good potential in treating asymptomatic MAFLD. Trial registration . The trial was registered in the US Library of Medicine (clinicaltrials.gov) with the number NCT06183801 on December 28, 2023.