Transcriptomic Analysis of Tea Plant Responding to Drought Stress and Recovery

• Published in: PloS one Vol. 11; no. 1; p. e0147306
• Main Authors: Liu, Sheng-Chuan, Jin, Ji-Qiang, Ma, Jian-Qiang, Yao, Ming-Zhe, Ma, Chun-Lei, Li, Chun-Fang, Ding, Zhao-Tang, Chen, Liang
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.01.2016; Public Library of Science (PLoS)
• Subjects: Abscisic acid; Acetic acid; Agriculture; Arabidopsis; Biomarkers - analysis; Biosynthesis; Breeding; Camellia sinensis - genetics; Camellia sinensis - growth & development; Crop yield; Crops; Cultivars; Drought; Drought resistance; Droughts; Economic conditions; Economic importance; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene sequencing; Genes; Genetic aspects; Genomes; Genomics; High-Throughput Nucleotide Sequencing - methods; Indoleacetic acid; Jasmonic acid; Kinases; Laboratories; Mannitol; Metabolism; Molecular modelling; Physiology; Plant biology; Plant breeding; Proline; Protein kinase; Proteins; Real-Time Polymerase Chain Reaction; Recovery; Recovery (Medical); Ribonucleic acid; RNA; RNA, Plant - genetics; Salicylic acid; Signal transduction; Signaling; Starch; Stomata; Stress (Physiology); Stress, Physiological - genetics; Sucrose; Sugar; Tea; Tea (Plant); Transcription factors; Trehalose; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0147306

Tea plant (Camellia sinensis) is an economically important beverage crop. Drought stress (DS) seriously limits the growth and development of tea plant, thus affecting crop yield and quality. To elucidate the molecular mechanisms of tea plant responding to DS, we performed transcriptomic analysis of tea plant during the three stages [control (CK) and during DS, and recovery (RC) after DS] using RNA sequencing (RNA-Seq). Totally 378.08 million high-quality trimmed reads were obtained and assembled into 59,674 unigenes, which were extensively annotated. There were 5,955 differentially expressed genes (DEGs) among the three stages. Among them, 3,948 and 1,673 DEGs were up-regulated under DS and RC, respectively. RNA-Seq data were further confirmed by qRT-PCR analysis. Genes involved in abscisic acid (ABA), ethylene, and jasmonic acid biosynthesis and signaling were generally up-regulated under DS and down-regulated during RC. Tea plant potentially used an exchange pathway for biosynthesis of indole-3-acetic acid (IAA) and salicylic acid under DS. IAA signaling was possibly decreased under DS but increased after RC. Genes encoding enzymes involved in cytokinin synthesis were up-regulated under DS, but down-regulated during RC. It seemed probable that cytokinin signaling was slightly enhanced under DS. In total, 762 and 950 protein kinases belonging to 26 families were differentially expressed during DS and RC, respectively. Overall, 547 and 604 transcription factor (TF) genes belonging to 58 families were induced in the DS vs. CK and RC vs. DS libraries, respectively. Most members of the 12 TF families were up-regulated under DS. Under DS, genes related to starch synthesis were down-regulated, while those related to starch decomposition were up-regulated. Mannitol, trehalose and sucrose synthesis-related genes were up-regulated under DS. Proline was probably mainly biosynthesized from glutamate under DS and RC. The mechanism by which ABA regulated stomatal movement under DS and RC was partly clarified. These results document the global and novel responses of tea plant during DS and RC. These data will serve as a valuable resource for drought-tolerance research and will be useful for breeding drought-resistant tea cultivars.