Role of the human endogenous retrovirus HERV-K18 in autoimmune disease susceptibility: study in the Spanish population and meta-analysis

• Published in: PloS one Vol. 8; no. 4; p. e62090
• Main Authors: de la Hera, Belén, Varadé, Jezabel, García-Montojo, Marta, Lamas, José Ramón, de la Encarnación, Ana, Arroyo, Rafael, Fernández-Gutiérrez, Benjamín, Alvarez-Lafuente, Roberto, Urcelay, Elena
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.04.2013; Public Library of Science (PLoS)
• Subjects: Adult; Antibodies; Antigens; Arthritis; Autoimmune diseases; Autoimmune Diseases - virology; Biology; Chromosome 1; Chromosomes; Development and progression; Diabetes; Diabetes mellitus; Disease susceptibility; Disease Susceptibility - virology; Drb1 protein; Endogenous retroviruses; Endogenous Retroviruses - genetics; Endogenous Retroviruses - physiology; Etiology; Female; Gene sequencing; Genetic aspects; Genomes; Genomics; Haplotypes; Health aspects; Heritability; Histocompatibility antigen HLA; HLA antigens; Humans; Immunologic factors; Immunology; Infection; Male; Medical research; Medicine; Meta-analysis; Middle Aged; Multiple sclerosis; Mutation; Neurology; Patients; Physiology; Population studies; Proteins; Rheumatoid arthritis; Rheumatology; Single-nucleotide polymorphism; Spain; Stem cells; Spain
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0062090

Human endogenous retroviruses (HERVs) are genomic sequences that resulted from ancestral germ-line infections by exogenous retroviruses and therefore are transmitted in a Mendelian fashion. Increased HERV expression and antibodies to HERV antigens have been found in various autoimmune diseases. HERV-K18 in chromosome 1 was previously associated with type one diabetes and multiple sclerosis (MS). The etiology of these complex conditions has not been completely elucidated even after the powerful genome wide association studies (GWAS) performed. Nonetheless, this approach does not scrutinize the repetitive sequences within the genome, and part of the missing heritability could lie behind these sequences. We aimed at evaluating the role of HERV-K18 in chromosome 1 on autoimmune disease susceptibility. Two HERV-K18 SNPs (97Y/C and 154W/Stop substitutions) conforming three haplotypes were genotyped in Spanish cohorts of multiple sclerosis (n = 942), rheumatoid arthritis (n = 462) and ethnically matched controls (n = 601). Our findings were pooled in a meta-analysis including 5312 autoimmune patients and 4032 controls. Significant associations of both HERV-K18 polymorphisms in chromosome 1 with MS patients stratified by HLA-DRB1*15:01 were observed [97Y/C p = 0.02; OR (95% CI) = 1.5 (1.04-2.17) and 154W/Stop: p = 0.001; OR (95% CI) = 1.6 (1.19-2.16)]. Combined meta-analysis of the previously published association studies of HERV-K18 with different autoimmune diseases, together with data derived from Spanish cohorts, yielded a significant association of the HERV-K18.3 haplotype [97Y-154W: p(M-H) = 0.0008; OR(M-H) (95% CI) = 1.22 (1.09-1.38)]. Association of the HERV-K18.3 haplotype in chromosome 1 with autoimmune-disease susceptibility was confirmed through meta-analysis.