Downregulation of PAX6 by shRNA inhibits proliferation and cell cycle progression of human non-small cell lung cancer cell lines

• Published in: PloS one Vol. 9; no. 1; p. e85738
• Main Authors: Zhao, Xiaoting, Yue, Wentao, Zhang, Lina, Ma, Li, Jia, Wenyun, Qian, Zhe, Zhang, Chunyan, Wang, Yue
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.01.2014; Public Library of Science (PLoS)
• Subjects: Apoptosis; Biology; Biotechnology; Cancer; Carcinoma, Non-Small-Cell Lung - metabolism; Cell cycle; Cell Cycle Checkpoints; Cell growth; Cell Line, Tumor; Cell Proliferation; Cellular biology; Cloning; Cyclin D1; Cyclin D1 - genetics; Cyclin D1 - metabolism; Cyclin-dependent kinases; D1 protein; Development and progression; Down-regulation; Embryos; Eye Proteins - genetics; Eye Proteins - metabolism; Female; Gene Expression; Gene Knockdown Techniques; Genes; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Hospitals; Humans; Infections; Kinases; Lung cancer; Lung diseases; Lung Neoplasms - metabolism; Male; MAP kinase; MAP Kinase Signaling System; Medicine; Middle Aged; Non-small cell lung cancer; Non-small cell lung carcinoma; Paired Box Transcription Factors - genetics; Paired Box Transcription Factors - metabolism; Pancreatic cancer; Patients; Pax6 protein; PAX6 Transcription Factor; Phosphorylation; Prostate; Protein Processing, Post-Translational; Repressor Proteins - genetics; Repressor Proteins - metabolism; Retinoblastoma Protein - metabolism; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - genetics; S Phase; siRNA; Small cell lung cancer; Stem cells; Tissues; Transcription factors; Tumor cell lines; Tumorigenesis; Tumors; Vascular endothelial growth factor; Beijing China; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0085738

The transcription factor PAX6 is primarily expressed in embryos. PAX6 is also expressed in several tumors and plays an oncogenic role. However, little is known about the role of PAX6 in lung cancer. The function of PAX6 in lung cancer cells was evaluated by small interfering RNA-mediated depletion of the protein followed by analyses of cell proliferation, anchorage-independent growth, and cell cycle arrest. The changes of cyclin D1, pRB, ERK1/2, p38 expression caused by PAX6 inhibition were detected using western-blotting. The PAX6 mRNA level in 52 pairs of tumors and corresponding matched adjacent normal tissues from non-small cell lung cancer patients and lung cancer cell lines was detected by real-time PCR. Suppression of PAX6 expression inhibited cell growth and colony formation in A549 and H1299 cells. The percentage of cells in G1-phase increased when PAX6 expression was inhibited. The cyclin D1 protein level, as well as the pRB phosphorylation level, decreased as a result of PAX6 down-regulation. The activity of ERK1/2 and p38 was also suppressed in PAX6 knock-down cells. The PAX6 mRNA was highly expressed in lung cancer tissue and lung cancer cell lines. In most patients (about 65%), the relative ratio of PAX6 mRNA in primary NSCLC versus adjacent tissues exceeded 100. Our data implicated that PAX6 accelerates cell cycle progression by activating MAPK signal pathway. PAX6 mRNA levels were significantly elevated in primary lung cancer tissues compared to their matched adjacent tissues.