Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating

• Published in: PloS one Vol. 10; no. 9; p. e0137017
• Main Authors: Chang, Chih-Hao, Yeh, Shu-Yun, Lee, Bing-Heng, Chen, Chia-Jie, Su, Chiao-Tzu, Lin, Yen-Ting, Liu, Chien-Lin, Chen, Hsien-Yeh
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.09.2015; Public Library of Science (PLoS)
• Subjects: 3T3 Cells; Alizarin; Alkaline phosphatase; Alkaline Phosphatase - biosynthesis; Alkaline Phosphatase - metabolism; Aluminum; Animals; Anthraquinones - metabolism; Biocompatibility; Biomedical materials; Biomolecules; Biotechnology; Bone marrow; Bone morphogenetic protein 2; Bone Morphogenetic Protein 2 - chemistry; Bone morphogenetic proteins; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell Line; Chemical engineering; Chemical synthesis; Chemical vapor deposition; Chloride; Coating; Coatings; Conjugation; Gene expression; Genetic markers; Growth factors; Immobilization; Low temperature; Medicine; Mice; Orthopedics; Osteoblastogenesis; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis - drug effects; Osteogenesis - physiology; Phosphatases; Polymerization; Polymers - chemistry; Protective coatings; Proteins; Silicon wafers; Spectrum analysis; Succinimides - chemistry; Surface Properties - drug effects; Tissue engineering; Titanium; Transplants & implants; United States > US; Taiwan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0137017

The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a one-step conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.