Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides

• Published in: PloS one Vol. 14; no. 7; p. e0214033
• Main Authors: Guo, Boyang, Itami, Jun, Oikawa, Kazusato, Motoda, Yoko, Kigawa, Takanori, Numata, Keiji
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.07.2019; Public Library of Science (PLoS)
• Subjects: Analysis; Bacterial Proteins - administration & dosage; Bacterial Proteins - metabolism; Biology and Life Sciences; Callus; Cell walls; Cell-Penetrating Peptides - chemistry; Cell-Penetrating Peptides - metabolism; Deoxyribonucleic acid; DNA; Drug Carriers - chemistry; Drug Carriers - metabolism; Drug Delivery Systems; Fluorescence; Genomes; Ions - chemistry; Ions - metabolism; Lipid bilayers; Lipid Bilayers - metabolism; Lipids; Luminescent Proteins - administration & dosage; Luminescent Proteins - metabolism; Membrane lipids; Models, Molecular; Nanomaterials; Oligopeptides - chemistry; Oligopeptides - metabolism; Oryza - metabolism; Peptides; Physical Sciences; Physicochemical properties; Protein binding; Proteins; Research and Analysis Methods; Rice; Seeds; Vacuum; Yellow fluorescent protein; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0214033

Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus.