Effects of Host Phylogeny and Habitats on Gut Microbiomes of Oriental River Prawn (Macrobrachium nipponense)

• Published in: PloS one Vol. 10; no. 7; p. e0132860
• Main Authors: Tzeng, Tzong-Der, Pao, Yueh-Yang, Chen, Po-Cheng, Weng, Francis Cheng-Hsuan, Jean, Wen Dar, Wang, Daryi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.07.2015; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Aquaculture; Bacteria; Biodiversity; Cardiovascular disease; Cellulose; Clustering; Communities; Community composition; Crustacea - microbiology; Digestive system; Digestive tract; Divergence; Ecosystem; Ecosystems; Fresh water; Freshwater environments; Gastrointestinal tract; Genetics; Habitats; Immune system; Intestinal microflora; Intestine; Intestines - microbiology; Lakes; Life sciences; Macrobrachium nipponense; Microbiomes; Microbiota; Microorganisms; National parks; Phylogeny; Populations; Rivers; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sibling species; Species; Studies; Taxonomy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0132860

The gut microbial community is one of the richest and most complex ecosystems on earth, and the intestinal microbes play an important role in host development and health. Next generation sequencing approaches, which rapidly produce millions of short reads that enable the investigation on a culture independent basis, are now popular for exploring microbial community. Currently, the gut microbiome in fresh water shrimp is unexplored. To explore gut microbiomes of the oriental river prawn (Macrobrachium nipponense) and investigate the effects of host genetics and habitats on the microbial composition, 454 pyrosequencing based on the 16S rRNA gene were performed. We collected six groups of samples, including M. nipponense shrimp from two populations, rivers and lakes, and one sister species (M. asperulum) as an out group. We found that Proteobacteria is the major phylum in oriental river prawn, followed by Firmicutes and Actinobacteria. Compositional analysis showed microbial divergence between the two shrimp species is higher than that between the two populations of one shrimp species collected from river and lake. Hierarchical clustering also showed that host genetics had a greater impact on the divergence of gut microbiome than host habitats. This finding was also congruent with the functional prediction from the metagenomic data implying that the two shrimp species still shared the same type of biological functions, reflecting a similar metabolic profile in their gut environments. In conclusion, this study provides the first investigation of the gut microbiome of fresh water shrimp, and supports the hypothesis of host species-specific signatures of bacterial community composition.