The impact of rumen-protected amino acids on the expression of key- genes involved in the innate immunity of dairy sheep

• Published in: PloS one Vol. 15; no. 5; p. e0233192
• Main Authors: Tsiplakou, Eleni, Mavrommatis, Alexandros, Skliros, Dimitrios, Righi, Federico, Flemetakis, Emmanouil
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.05.2020; Public Library of Science (PLoS)
• Subjects: Alfalfa; Amino acid supplements; Amino acids; Animal sciences; Animals; Biology and Life Sciences; Biotechnology; Cytokines; Dairy cattle; Diet; Dietary supplements; Feed additives; Food and nutrition; Gene expression; Genes; Genetic aspects; Health aspects; Immune system; Immunity; Inflammation; Innate immunity; Interferon regulatory factor 3; Interleukin 10; JunD gene; Kinases; Laboratories; Leukocytes (neutrophilic); Levels; Lysine; MAP kinase; Medicine and Health Sciences; Messenger RNA; Metabolism; Methionine; Milk; Molecular biology; Monocytes; MyD88 protein; Natural immunity; Neutrophils; Nutrition; Ovis aries; Pathogens; Physical Sciences; Physiology; Proteins; RNA; Rumen; Sheep; Signal transduction; Stat3 protein; Transcription; Veterinary dietary supplements; Wheat straw; Zoology; Italy; Greece
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0233192

Rumen protected amino acids inclusion in ewes' diets has been proposed to enhance their innate immunity. The objective of this work was to determine the impact of dietary supplementation with rumen-protected methionine or lysine, as well as with a combination of these amino acids in two different ratios, on the expression of selected key-genes (NLRs, MyD88, TRIF, MAPK-1, IRF-3, JunD, TRAF-3, IRF-5, IL-1α, IL-10, IKK-α, STAT-3 and HO-1). Thus, sixty Chios dairy ewes (Ovis aries) were assigned to one of the following five dietary treatments (12 animals/ treatment): A: basal diet consist of concentrates, wheat straw and alfalfa hay (control group); B: basal diet +6.0 g/head rumen-protected methionine; C: basal diet + 5.0 g/head rumen-protected lysine; D: basal diet +6.0 g/head rumen-protected methionine + 5.0 g/head rumen-protected lysine and E: basal diet +12.0 g/head rumen-protected methionine + 5.0 g/head rumen-protected lysine. The results revealed a significant downregulation of relative transcript level of the IL-1α gene in the neutrophils of C and in monocytes of D ewes compared with the control. Significantly lower mRNA transcript accumulation was also observed for the MyD88 gene in the neutrophils of ewes fed with lysine only (C). The mRNA relative expression levels of JunD gene were highly induced in the monocytes, while those of IL-10 and HO-1 genes were declined in the neutrophils of ewes fed with the C and D diets compared with the control. Lower transcript levels of STAT-3 gene were observed in the neutrophils of ewes fed with either C or with E diets in comparison with the control. In conclusion, our results suggest that the dietary supplementation of ewes with rumen-protected amino acids, down regulate the expression of some genes involved in the pro-inflammatory signalling.