A non-destructive DNA sampling technique for herbarium specimens

Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for e...

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Bibliographic Details
Published inPloS one Vol. 12; no. 8; p. e0183555
Main Author Shepherd, Lara D
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 31.08.2017
Public Library of Science (PLoS)
Subjects
Asplenium
Biology and Life Sciences
Chloroplasts
Deoxyribonucleic acid
DNA
DNA sequencing
DNA, Plant - genetics
DNA, Plant - isolation & purification
Earth sciences
Endangered & extinct species
Extinction
Herbaria
Laboratories
Leaves
Methods
Mitochondrial DNA
Museums
Nondestructive testing
Plant Leaves - genetics
Plants - genetics
Polymerase Chain Reaction
Rare species
Research and analysis methods
Sampling
Sampling methods
Sampling techniques
Sequence Analysis, DNA
Specimen Handling
Stems
New Zealand
United States > US
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Summary:Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for extracting DNA from herbarium specimens. DNA was successfully retrieved from robust leaves and/or stems of herbarium specimens up to 73 years old.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Competing Interests: The author has declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0183555