Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

• Published in: PloS one Vol. 14; no. 9; p. e0221457
• Main Authors: Winnier, Glenn E, Valenzuela, Nick, Peters-Hall, Jennifer, Kellner, Joshua, Alt, Christopher, Alt, Eckhard U
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03.09.2019; Public Library of Science (PLoS)
• Subjects: Adipose tissue; Adult; Arthritis; Biology and Life Sciences; Body fat; Bone marrow; Cell Count; Cell Differentiation; Cell Separation - methods; Cell Survival; Cell suspensions; Cell viability; Cell- and Tissue-Based Therapy; Enzymes; Female; Gene Expression; Genetic aspects; Humans; Indicators and Reagents; Lipectomy; Liposuction; Literature reviews; Male; Medical devices; Medical electronics; Medical equipment; Medicine and Health Sciences; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - physiology; Methods; Middle Aged; Physiological aspects; Physiology; Reagents; Regenerative Medicine; Research and Analysis Methods; Rotator cuff; Standard deviation; Stem cells; Stem Cells - cytology; Stem Cells - physiology; Subcutaneous Fat - cytology; Yield; Germany; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0221457

Freshly isolated, uncultured, autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell viability, live cell yield, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 times more colony forming units. Despite these differences, cells isolated from lipoaspirate both with and without the use of Matrase Reagent were independently able to differentiate into cells of all three germ layers. This indicates that biological characteristics, physiological functions or structural properties relevant for the intended use were not altered or induced using Matrase Reagent. A comprehensive literature review demonstrated that isolation of ADRCs from lipoaspirate using the Transpose RT system and the Matrase Reagent results in the highest viable cell yield among published data regarding isolation of ADRCs from lipoaspirate.