Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species

• Published in: PloS one Vol. 11; no. 8; p. e0160544
• Main Authors: Mendonça, Marcelo, Moreira, Gustavo Marçal Schmidt Garcia, Conceição, Fabricio Rochedo, Hust, Michael, Mendonça, Karla Sequeira, Moreira, Ângela Nunes, França, Rodrigo Correa, da Silva, Wladimir Padilha, Bhunia, Arun K, Aleixo, José Antonio G
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.08.2016; Public Library of Science (PLoS)
• Subjects: Aldolase; Aldolases; Amino Acid Sequence; Amino acids; Antibodies, Monoclonal - immunology; Antigenic determinants; Antigens; Antigens, Surface - immunology; Antigens, Surface - metabolism; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacterial Proteins - metabolism; Biology and Life Sciences; Blotting, Western; Catalytic Domain; Cell adhesion; Cell surface; Cell walls; Cheese; Cheese - microbiology; Cloning; Cloning, Molecular; Dairy products; Diagnostic systems; Dimerization; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes - immunology; Food contamination & poisoning; Food Microbiology; Food production; Food science; Fructose; Fructose-1,6-diphosphate; Fructose-Bisphosphate Aldolase - genetics; Fructose-Bisphosphate Aldolase - immunology; Fructose-Bisphosphate Aldolase - metabolism; Gene mapping; Genetic aspects; Immunogenicity; Immunoglobulins; Laboratories; Listeria; Listeria - enzymology; Listeria - immunology; Listeria - isolation & purification; Listeria monocytogenes; Localization; Mass Spectrometry; Mass spectroscopy; Medicine and Health Sciences; Monoclonal antibodies; Pathogens; Peptide mapping; Peptides - analysis; Physiological aspects; Protein Structure, Tertiary; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - immunology; Recombinant Proteins - isolation & purification; Research and Analysis Methods; Streptococcus infections; Target detection; Brazil

Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work...