Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species

• Published in: PloS one Vol. 11; no. 8; p. e0160544
• Main Authors: Mendonça, Marcelo, Moreira, Gustavo Marçal Schmidt Garcia, Conceição, Fabricio Rochedo, Hust, Michael, Mendonça, Karla Sequeira, Moreira, Ângela Nunes, França, Rodrigo Correa, da Silva, Wladimir Padilha, Bhunia, Arun K, Aleixo, José Antonio G
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.08.2016; Public Library of Science (PLoS)
• Subjects: Aldolase; Aldolases; Amino Acid Sequence; Amino acids; Antibodies, Monoclonal - immunology; Antigenic determinants; Antigens; Antigens, Surface - immunology; Antigens, Surface - metabolism; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacterial Proteins - metabolism; Biology and Life Sciences; Blotting, Western; Catalytic Domain; Cell adhesion; Cell surface; Cell walls; Cheese; Cheese - microbiology; Cloning; Cloning, Molecular; Dairy products; Diagnostic systems; Dimerization; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes - immunology; Food contamination & poisoning; Food Microbiology; Food production; Food science; Fructose; Fructose-1,6-diphosphate; Fructose-Bisphosphate Aldolase - genetics; Fructose-Bisphosphate Aldolase - immunology; Fructose-Bisphosphate Aldolase - metabolism; Gene mapping; Genetic aspects; Immunogenicity; Immunoglobulins; Laboratories; Listeria; Listeria - enzymology; Listeria - immunology; Listeria - isolation & purification; Listeria monocytogenes; Localization; Mass Spectrometry; Mass spectroscopy; Medicine and Health Sciences; Monoclonal antibodies; Pathogens; Peptide mapping; Peptides - analysis; Physiological aspects; Protein Structure, Tertiary; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - immunology; Recombinant Proteins - isolation & purification; Research and Analysis Methods; Streptococcus infections; Target detection; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160544

Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (FBA) class II in Listeria species is the antigen target of the previously described mAb-3F8. Western and dot blot assays confirmed that the mAb-3F8 could distinguish all tested Listeria species from close-related bacteria. Localization studies indicated that FBA is present in every fraction of Listeria cells, including supernatant and the cell wall, setting Listeria spp. as one of the few bacteria described to have this protein on their cell surface. Epitope mapping using ORFeome display and a peptide membrane revealed a 14-amino acid peptide as the potential mAb-3F8 epitope. The target epitope in FBA allowed distinguishing Listeria spp. from closely-related bacteria, and was identified as part of the active site in the dimeric enzyme. However, its function in cell surface seems not to be host cell adhesion-related. Western and dot blot assays further demonstrated that mAb-3F8 together with anti-InlA mAb-2D12 could differentiate pathogenic from non-pathogenic Listeria isolated from artificially contaminated cheese. In summary, we report FBA as a novel immunogenic surface target useful for the detection of Listeria genus.