Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype

• Published in: PloS one Vol. 17; no. 5; p. e0268073
• Main Authors: Ciereszko, Andrzej, Dietrich, Mariola A, Słowińska, Mariola, Nynca, Joanna, Ciborowski, Michał, Kaczmarek, Monika M, Myszczyński, Kamil, Kiśluk, Joanna, Majewska, Anna, Michalska-Falkowska, Anna, Kodzik, Natalia, Reszeć, Joanna, Sierko, Ewa, Nikliński, Jacek
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.05.2022; Public Library of Science (PLoS)
• Subjects: Adenocarcinoma; Adenocarcinoma - pathology; Adherens junctions; Analysis; Angiogenesis; Antigens; Biology and Life Sciences; Biomarkers; Biomarkers, Tumor - metabolism; Biosynthesis; Breast cancer; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Squamous Cell - pathology; Care and treatment; Cell migration; Cytoskeleton; Dehydrogenases; Diagnosis; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; FDA approval; Gel electrophoresis; Humans; Hypoxia; Hypoxia-inducible factors; Inflammation; Keratin; Lung cancer; Lung cancer, Non-small cell; Lung carcinoma; Lung diseases; Lung Neoplasms - pathology; Margins of Excision; Medicine and Health Sciences; Metabolism; Mortality; Non-small cell lung carcinoma; Protein folding; Protein structure; Protein turnover; Proteins; Proteomics; Research and Analysis Methods; Signal transduction; Signaling; Small cell lung carcinoma; Squamous cell carcinoma; Thyroid; Thyroid cancer; Tumors; Two-Dimensional Difference Gel Electrophoresis; Poland
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0268073

Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients taken from the tumor center and tumor margin. Two subtypes of NSCLC, adenocarcinoma (ADC) and squamous cell carcinoma (SCC) were compared. Data are available via ProteomeXchange with identifier PXD032736 and PXD032962 for ADC and SCC, respectively. For ADC proteins, 26 significant canonical pathways were identified, including Rho signaling pathways, a semaphorin neuronal repulsive signaling pathway, and epithelial adherens junction signaling. For SCC proteins, nine significant canonical pathways were identified, including hypoxia-inducible factor-1α signaling, thyroid hormone biosynthesis, and phagosome maturation. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, EMC transition, epithelial adherens junctions, and inflammatory responses. In conclusion, we identified several proteins that are important for the better characterization of tumor development and molecular specificity of both lung cancer subtypes. We also identified proteins that may be important as biomarkers and/or targets for anticancer therapy.