Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice

• Published in: PloS one Vol. 12; no. 8; p. e0181365
• Main Authors: Oshota, Olusegun, Conway, Max, Fookes, Maria, Schreiber, Fernanda, Chaudhuri, Roy R, Yu, Lu, Morgan, Fiona J E, Clare, Simon, Choudhary, Jyoti, Thomson, Nicholas R, Lio, Pietro, Maskell, Duncan J, Mastroeni, Pietro, Grant, Andrew J
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.08.2017; Public Library of Science (PLoS)
• Subjects: Animals; Bacteria; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Bioinformatics; Biology and Life Sciences; Cell division; Cell survival; Disseminated infection; Environmental conditions; Exposure; Female; Fitness; Gene Deletion; Gene expression; Gene Expression Regulation, Bacterial; Gene regulation; Genes, Bacterial; Genomes; Growth rate; Health aspects; Immune response; Immune system; Immunologic Deficiency Syndromes - genetics; Immunologic Deficiency Syndromes - microbiology; In vitro methods and tests; Infections; Laboratories; Male; Medicine and Health Sciences; Metabolic flux; Metabolism; Mice; Mice, Inbred C57BL; Phagocytes; Prevention; Proteins; Proteome - analysis; Proteome - genetics; Proteomes; Proteomics; Public health; Receptors, Immunologic - analysis; Receptors, Immunologic - genetics; Risk factors; Salmonella; Salmonella enterica; Salmonella Infections, Animal - genetics; Salmonella Infections, Animal - microbiology; Salmonella typhimurium - genetics; Salmonellosis; Tissues; Transcription (Genetics); Transcriptome; Typhoid; Vaccines; Veterinary medicine; Viral vaccines; Virulence; United Kingdom > UK; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0181365

Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S. enterica adapts in vivo to sustain cell division and survival. We have studied the transcriptome, proteome and metabolic flux of Salmonella, and the transcriptome of the host during infection of wild type C57BL/6 and immune-deficient gp91-/-phox mice. Our analyses advance the understanding of how S. enterica and the host behaves during infection to a more sophisticated level than has previously been reported.