Hydrogen sulfide promotes adipogenesis in 3T3L1 cells

• Published in: PloS one Vol. 10; no. 3; p. e0119511
• Main Authors: Tsai, Chin-Yi, Peh, Meng Teng, Feng, Wei, Dymock, Brian William, Moore, Philip Keith
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.03.2015; Public Library of Science (PLoS)
• Subjects: 3-Mercaptopyruvate sulfurtransferase; 3T3-L1 Cells; Adipocytes; Adipocytes - cytology; Adipocytes - drug effects; Adipocytes - metabolism; Adipogenesis; Adipogenesis - drug effects; Adipogenesis - genetics; Adipogenesis - physiology; Animals; Cardiovascular disease; Cell Differentiation - drug effects; Cystathionine beta-Synthase - genetics; Cystathionine beta-Synthase - metabolism; Cystathionine gamma-Lyase - genetics; Cystathionine gamma-Lyase - metabolism; Diabetes; Differentiation; Enzymes; Fatty acid-binding protein; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Fatty acids; Gene expression; Gene Expression - drug effects; Genes; Glycerol; Glycerol - metabolism; Hydrogen; Hydrogen ion concentration; Hydrogen sulfide; Hydrogen Sulfide - metabolism; Hydrogen Sulfide - pharmacology; Inhibitors; Insulin resistance; Life sciences; Lipid Metabolism - drug effects; Lipids; Lipolysis; Lipolysis - drug effects; Metabolism; Metabolites; Mice; Morpholines - pharmacology; Neurobiology; Neurosciences; Obesity; Organothiophosphorus Compounds - pharmacology; Penicillin; Pharmacology; Pharmacy; Protein binding; Proteins; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-mediated interference; siRNA; Sodium; Sulfides - pharmacology; Sulfurtransferase; Sulfurtransferases - genetics; Sulfurtransferases - metabolism; Time dependence; United States > US; Singapore
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0119511

The effect of hydrogen sulfide (H2S) on differentiation of 3T3L1-derived adipocytes was examined. Endogenous H2S was increased after 3T3L1 differentiation. The expression of the H2S-synthesising enzymes, cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (3-MST), was increased in a time-dependent manner during 3T3L1 differentiation. Expression of genes associated with adipogenesis related genes including fatty acid binding protein 4 (FABP4/aP2), a key regulator of this process, was increased by GYY4137 (a slow-releasing H2S donor compound) and sodium hydrosulfide (NaHS, a classical H2S donor) but not by ZYJ1122 or time-expired NaHS. Furthermore expression of these genes were reduced by aminooxyacetic acid (AOAA, CBS inhibitor), DL-propargylglycine (PAG, CSE inhibitor) as well as by CSE small interference RNA (siCSE) and siCBS. The size and number of lipid droplets in mature adipocytes was significantly increased by both GYY4137 and NaHS, which also impaired the ability of CL316,243 (β3-agonist) to promote lipolysis in these cells. In contrast, AOAA and PAG had the opposite effect. Taken together, we show that the H2S-synthesising enzymes CBS, CSE and 3-MST are endogenously expressed during adipogenesis and that both endogenous and exogenous H2S modulate adipogenesis and adipocyte maturation.