A genetic variant in the promoter region of miR-106b-25 cluster predict clinical outcome of HBV-related hepatocellular carcinoma in Chinese

• Published in: PloS one Vol. 9; no. 1; p. e85394
• Main Authors: Qi, Fuzhen, Huang, Mingde, Pan, Yun, Liu, Yao, Liu, Jibin, Wen, Juan, Xie, Kaipeng, Shen, Hongbing, Ma, Hongxia, Miao, Yi, Hu, Zhibin
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.01.2014; Public Library of Science (PLoS)
• Subjects: Asian Continental Ancestry Group; Biomarkers; Carcinoma, Hepatocellular - ethnology; Carcinoma, Hepatocellular - etiology; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - mortality; Cell adhesion & migration; Cell cycle; Clusters; Confidence intervals; Epidemiology; Fatalities; Female; Gene polymorphism; Genetic diversity; Genetic variance; Genotypes; Hepatitis; Hepatitis B; Hepatitis B - complications; Hepatitis B - ethnology; Hepatitis B - genetics; Hepatitis B - mortality; Hepatocellular carcinoma; Hospitals; Humans; Immune response; Immune system; Introns; Laboratories; Liver cancer; Liver Neoplasms - ethnology; Liver Neoplasms - etiology; Liver Neoplasms - genetics; Liver Neoplasms - mortality; Lung cancer; Male; Medical prognosis; Medicine; MicroRNAs; MicroRNAs - genetics; Middle Aged; Minichromosome Maintenance Complex Component 7 - genetics; Neoplasm Staging; Patient outcomes; Patients; Polymorphism; Polymorphism, Single Nucleotide; Prevention; Prognosis; Promoter Regions, Genetic; Proportional Hazards Models; Public health; Regression analysis; Reproductive health; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Statistical analysis; Surgery; Survival Analysis; Tumorigenesis; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0085394

MiR-106b-25 cluster, hosted in intron 13 of MCM7, may play integral roles in diverse processes including immune response, tumorigenesis and progression. A single nucleotide polymorphism (SNP), rs999885, is located in the promoter region of MCM7. Our previous study showed that the A to G base change of rs999885 may provide an increased risk for HCC in HBV persistent carriers by altering the expression of the miR-106b-25 cluster. However, it is unknown whether rs999885 is associated with prognosis of intermediate or advanced HBV-related hepatocellular carcinoma (HCC) patients. The SNP, rs999885, was genotyped by using the TaqMan allelic discrimination Assay in 414 intermediate or advanced HCC patients. Log-rank test and Cox proportional hazard models were used for survival analysis. The variant genotypes of rs999885 were associated with a significantly decreased risk of death for intermediate or advanced HCC [additive model: adjusted hazard ratio (HR)  = 0.76,95% confidence intervals (CI)  = 0.59-0.97]. Further stepwise regression analysis suggested that rs999885 was an independently protective factor for the prognosis of HCC in the final model (additive model: adjusted HR  = 0.72, 95% CI  = 0.56-0.91, P = 0.007). These findings indicate that the A to G base change of rs999885 may provide a protective effect on the prognosis of intermediate or advanced HCC in Chinese.