Oleic acid and octanoic acid sensing capacity in rainbow trout Oncorhynchus mykiss is direct in hypothalamus and Brockmann bodies

• Published in: PloS one Vol. 8; no. 3; p. e59507
• Main Authors: Librán-Pérez, Marta, López-Patiño, Marcos A, Míguez, Jesús M, Soengas, José L
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.03.2013; Public Library of Science (PLoS)
• Subjects: Amino acids; Animals; Biology; Caprylates - pharmacology; Chains; Detection; Fatty acids; Fatty Acids - metabolism; Fish; Fishes; Food intake; Hypothalamus; Hypothalamus - drug effects; Hypothalamus - metabolism; Inhibitors; Kinases; Metabolism; Monounsaturated fatty acids; Neuroendocrine system; Neuropeptides; Nuclear receptors; Octanoic acid; Oleic acid; Oleic Acid - pharmacology; Oncorhynchus mykiss; Oncorhynchus mykiss - metabolism; Oxidation; Oxygen; Parameters; Reactive oxygen species; Receptors; Salmon; Saturated fatty acids; Transcription factors; Trout; Spain
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0059507

In a previous study, we provided evidence for the presence in hypothalamus and Brockmann bodies (BB) of rainbow trout Oncorhynchus mykiss of sensing systems responding to changes in levels of oleic acid (long-chain fatty acid, LCFA) or octanoic acid (medium-chain fatty acid, MCFA). Since those effects could be attributed to an indirect effect, in the present study, we evaluated in vitro if hypothalamus and BB respond to changes in FA in a way similar to that observed in vivo. In a first set of experiments, we evaluated in hypothalamus and BB exposed to increased oleic acic or octanoic acid concentrations changes in parameters related to FA metabolism, FA transport, nuclear receptors and transcription factors, reactive oxygen species (ROS) effectors, components of the KATP channel, and (in hypothalamus) neuropeptides related to food intake. In a second set of experiments, we evaluated in hypothalamus the response of those parameters to oleic acid or octanoic acid in the presence of inhibitors of fatty acid sensing components. The responses observed in vitro in hypothalamus are comparable to those previously observed in vivo and specific inhibitors counteracted in many cases the effects of FA. These results support the capacity of rainbow trout hypothalamus to directly sense changes in MCFA or LCFA levels. In BB increased concentrations of oleic acid or octanoic acid induced changes that in general were comparable to those observed in hypothalamus supporting direct FA sensing in this tissue. However, those changes were not coincident with those observed in vivo allowing us to suggest that the FA sensing capacity of BB previously characterized in vivo is influenced by other neuroendocrine systems.