The effect of platelet-rich plasma on osseous healing in dogs undergoing high tibial osteotomy
The purpose of this study was to investigate whether platelet-rich plasma (PRP) enhances osseous healing in conjunction with a high tibial osteotomy in dogs. Randomized controlled trial. Sixty-four client-owned pet dogs with naturally occurring rupture of the anterior cruciate ligament and that were...
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Published in | PloS one Vol. 12; no. 5; p. e0177597 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Public Library of Science
16.05.2017
Public Library of Science (PLoS) |
Subjects | |
Online Access | Get full text |
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Summary: | The purpose of this study was to investigate whether platelet-rich plasma (PRP) enhances osseous healing in conjunction with a high tibial osteotomy in dogs.
Randomized controlled trial.
Sixty-four client-owned pet dogs with naturally occurring rupture of the anterior cruciate ligament and that were to be treated with a high tibial osteotomy (tibial plateau leveling osteotomy) were randomized into the treatment or control group. Dogs in the treatment group received autologous platelet-rich plasma activated with calcium chloride and bovine thrombin to produce a well-formed PRP gel that was placed into the osteotomy at the time of surgery. Dogs in the control group received saline lavage of the osteotomy. All dogs had the osteotomy stabilized with identical titanium alloy implants and all aspects of the surgical procedure and post-operative care were identical among dogs of the two groups. Bone healing was assessed at exactly 28, 49, and 70 days after surgery with radiography and ultrasonography and with MRI at day 28. The effect of PRP on bone healing was assessed using a repeated measures analysis of covariance with radiographic and ultrasonographic data and using a t-test with the MRI data.
Sixty dogs completed the study. There were no significant differences in age, weight, or gender distribution between the treatment and control groups. Twenty-seven dogs were treated with PRP and 33 were in the control group. The average platelet concentration of the PRP was 1.37x106 platelets/μL (±489x103) with a leukocyte concentration of 5.45x103/μL (±3.5x103). All dogs demonstrated progressive healing over time and achieved clinically successful outcomes. Time since surgery and patient age were significant predictors of radiographic healing and time since surgery was a significant predictor of ultrasonographic assessment of healing. There was no significant effect of PRP treatment as assessed radiographically, ultrasonographically, or with MRI.
The PRP used in this study did not hasten osseous union in dogs treated with a high tibial osteotomy. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: I do not believe my [Sam Franklin] relationship with Arthrex Vet Systems biased the results. In fact, as you review the manuscript you can see that we ultimately generated data that do not support the use of platelet-rich plasma for this specific application. Accordingly, it is clear that we are not providing data or results that are in the interest of this supporting company. Furthermore, Sam Franklin’s relationship or support from either Arthrex or the AO Foundation does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials. Conceptualization: SPF.Data curation: SPF EEB SPH.Formal analysis: SPF.Funding acquisition: SPF.Investigation: SPF EEB SPH.Methodology: SPF SPH.Project administration: SPF EEB SPH.Resources: SPF.Supervision: SPF SPH.Validation: SPF.Visualization: SPF EEB SPH.Writing – original draft: SPF.Writing – review & editing: SPF EEB SPH. |
ISSN: | 1932-6203 1932-6203 |
DOI: | 10.1371/journal.pone.0177597 |