Ureter regeneration-the proper scaffold has to be defined

• Published in: PloS one Vol. 9; no. 8; p. e106023
• Main Authors: Kloskowski, Tomasz, Jundziłł, Arkadiusz, Kowalczyk, Tomasz, Nowacki, Maciej, Bodnar, Magdalena, Marszałek, Andrzej, Pokrywczyńska, Marta, Frontczak-Baniewicz, Małgorzata, Kowalewski, Tomasz A, Chłosta, Piotr, Drewa, Tomasz
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.08.2014; Public Library of Science (PLoS)
• Subjects: Acids; Analysis; Animals; Aorta; Aorta, Thoracic; Aortic arch; Biology and Life Sciences; Bladder; Caprolactone; Cell adhesion & migration; Cells, Cultured; Chondrocytes - cytology; Chondrocytes - drug effects; Collagen; Defects; Endothelial Cells - cytology; Endothelial Cells - drug effects; Endothelial Cells - physiology; Engineering and Technology; Immunohistochemistry; Intravenous administration; Kopernik, Mikolaj (Nicolaus Copernicus) (1473-1543); Male; Medicine; Medicine and Health Sciences; Muscle, Smooth - drug effects; Muscle, Smooth - physiology; Muscles; Polyesters; Polyesters - chemical synthesis; Polyesters - pharmacology; Polymers; Rats; Rats, Wistar; Reconstruction; Regeneration; Regeneration - drug effects; Regeneration - physiology; Scaffolds; Smooth muscle; Surgery; Tissue engineering; Tissue Engineering - instrumentation; Tissue Engineering - methods; Tissue Scaffolds; Ureter; Ureter - surgery; Urine; Urography; Urology; Urothelium; Urothelium - drug effects; Urothelium - physiology; Poland; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0106023

The aim of this study was to compare two different acellular scaffolds: natural and synthetic, for urinary conduit construction and ureter segment reconstruction. Acellular aortic arch (AAM) and poly(L-lactide-co-caprolactone) (PLCL) were used in 24 rats for ureter reconstruction in both tested groups. Follow-up period was 4 weeks. Intravenous pyelography, histological and immunohistochemical analysis were performed. All animals survived surgical procedures. Patent uretero-conduit junction was observed only in one case using PLCL. In case of ureter segment reconstruction ureters were patent in one case using AAM and in four cases using PLCL scaffolds. Regeneration of urothelium layer and focal regeneration of smooth muscle layer was observed on both tested scaffolds. Obtained results indicates that synthetic acellular PLCL scaffolds showed better properties for ureter reconstruction than naturally derived acellular aortic arch.