Genetic analysis of 32 InDels in four ethnic minorities from Chinese Xinjiang

• Published in: PloS one Vol. 16; no. 4; p. e0250206
• Main Authors: Huang, Yujie, Chen, Xiaoying, Liu, Cong, Han, Xueli, Xiao, Chao, Yi, Shaohua, Huang, Daixin
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.04.2021; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Chromosomes; Computer and Information Sciences; Deoxyribonucleic acid; DNA; Editing; Forensic medicine; Forensic science; Genetic analysis; Genetic aspects; Genetic markers; Genetics; Genomes; Identification and classification; Insertion; Medical schools; Medicine; Medicine and Health Sciences; Minorities; Minority & ethnic groups; People and Places; Physical Sciences; Population; Principal components analysis; Research and Analysis Methods; Reviews; Science and technology; Technology; Tumors; China; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0250206

The present study used the previously constructed 32-plex InDels panel to investigated the genetic diversity of four ethnic minorities (Hui, Mongol, Uygur and Kazakh) from Xinjiang, and analyzed the genetic relationships between the four populations and 27 reference populations. No significant deviations were observed from the Hardy-Weinberg equilibrium (HWE) at the 32 InDels for each population. The average observed heterozygosity (Hexp), average polymorphic information content (PIC), combined power of discrimination (CPD) and cumulative probability of exclusion (CPE) for the 32 InDels were all higher than the Qiagen Investigator DIPplex kit in the four populations from Xinjiang. The CPD ranged from 0.999999999999903 (Kazakh) to 0.999999999999952 (Hui) and CPE ranged from 0.9971 (Uygur) to 0.9985 (Hui), which indicated that the 32 InDels were capable for individual identification and could be a supplementary tool in paternity test for these populations. Population genetic analysis by the method of analysis of molecular variance (AMOVA), FST, phylogenetic tree, TreeMix-based topology, multi-dimensional scale analysis (MDS), principal components analysis (PCA) and STRUCTURE analysis showed that Xinjiang Hui population has a close relationship with East Asians (EAS), especially Chinese Han, and the populations of Xinjiang Mongol, Uygur and Kazakh showed mixed ancestral components related to EAS and Europeans (EUR).