Bioinformatic analysis and clinical diagnostic value of hsa_circ_0004099 in acute ischemic stroke

• Published in: PloS one Vol. 17; no. 11; p. e0277832
• Main Authors: Zheng, Jiqing, Luo, Shuiming, Long, Yaobin
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.11.2022; Public Library of Science (PLoS)
• Subjects: Arteriosclerosis; Artificial intelligence; Bioinformatics; Biological markers; Biology and life sciences; Biomarkers; Biomarkers - metabolism; Computational Biology; Computer and Information Sciences; Correlation analysis; Data analysis; Data collection; Diabetes; Diagnosis; Diagnostic systems; Disease; Emergency medical care; Encyclopedias; FOXO3 protein; Gene expression; Genes; Genetic aspects; Genomes; Health aspects; Hospitals; Humans; Hypertension; Ischemia; Ischemic Stroke - diagnosis; Ischemic Stroke - genetics; K-Ras protein; Medical diagnosis; Medicine and Health Sciences; MicroRNAs; Neurological research; Pathogenesis; Plasma; Protein interaction; Proteins; Proto-Oncogene Proteins p21(ras) - metabolism; Research and Analysis Methods; RNA; RNA sequencing; RNA, Circular - genetics; Smad4 protein; Software; Stroke; Stroke (Disease); United States; Veins & arteries; United States; Taiwan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0277832

This study investigates the expression and effect of hsa_circ_0004099 in acute ischemic stroke (AIS). We conducted a case-controlled study that included 40 patients with AIS within 24 hours and 40 healthy subjects during the same period as a control group. Differentially expressed circular RNAs (circRNAs) were obtained using GEO2R, and the expression of hsa_circ_0004099 was verified using RT-PCR. Correlation analysis of the National Institutes of Health Stroke Scale (NIHSS) disease severity score and ischemic time with hsa_circ_0004099 expression levels was also performed. The receiver operating characteristic (ROC) curve of hsa_circ_0004099 was constructed, and bioinformatic analysis of hsa_circ_0004099 was performed. NIHSS scores negatively correlated with hsa_circ_0004099 levels (P<0.001, r = -0.7053), whereas infarct time was negatively correlated with hsa_circ_0004099 levels (P<0.001, r = -0.5130); hsa_circ_0004099 could benefit clinical diagnosis (area under the curve [AUC]: 0.923 [95% confidence interval [CI]: 0.8680–0.9904]). Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that hsa_circ_0004099 was enriched in several cancer pathways, which were collectively enriched in four genes namely TCF7L2, NRAS, CTNNB1, and KRAS. Eight core proteins were screened using a protein-protein interaction (PPI) network namely SMAD4, HIF1A, CTNNB1, CDKN1B, CDK6, FOXO3, KRAS, and NRAS. hsa_circ_0004099 is a potential clinical diagnostic marker. In addition, the possible role of hsa_circ_0004099 in the pathogenesis of AIS was analyzed using bioinformatics, which provided a new potential molecular target for AIS treatment.