Evaluation of Xpert MTB/RIF assay for detection of Mycobacterium tuberculosis in stool samples of adults with pulmonary tuberculosis

• Published in: PloS one Vol. 13; no. 9; p. e0203063
• Main Authors: Rahman, S. M. Mazidur, Maliha, Umme Tasnim, Ahmed, Shahriar, Kabir, Senjuti, Khatun, Razia, Shah, Javeed A., Banu, Sayera
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.09.2018; Public Library of Science (PLoS)
• Subjects: Adult; Adults; Aged; Aged, 80 and over; Antibiotics, Antitubercular - therapeutic use; Assaying; Bacilli; Bacteriological Techniques - methods; Biology and Life Sciences; Citric acid; Control; Correlation; Culture; Decontamination; Diagnosis; Disease transmission; Engineering and Technology; Feces - microbiology; Female; HIV; Human immunodeficiency virus; Humans; Infectious diseases; Laboratories; Lung diseases; Male; Medical diagnosis; Medicine and Health Sciences; Methods; Microbial Sensitivity Tests; Microscopy; Middle Aged; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Patient outcomes; Patients; Pediatrics; Research and Analysis Methods; Rifampin; Rifampin - therapeutic use; Risk factors; RpoB protein; Sensitivity; Sensitivity and Specificity; Smear; Sodium hydroxide; Specimen Handling; Sputum; Sputum - microbiology; Tuberculosis; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - drug therapy; Young Adult; United States > US; Bangladesh
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0203063

The Xpert MTB/RIF (Xpert) assay technology allows rapid and sensitive diagnosis of pulmonary tuberculosis (PTB) from sputum specimens. However, diagnosis of PTB is difficult for patients who cannot produce sputum. The objective of this study was to investigate the use of Xpert assay for successful detection of PTB using stool samples from adult subjects. Both stool and sputum samples from known smear and Xpert positive PTB patients were collected from a TB hospital in Dhaka. Stool samples were collected from healthy individuals without TB symptoms from a slum area of Dhaka. Stool and sputum samples were decontaminated and concentrated using NALC-NaOH-Na-citrate solution and the resultant sediment was used for Xpert, acid-fast bacilli (AFB) microscopy and culture. A total of 102 stool samples were collected from PTB patients and another 50 stool samples from healthy individuals without TB. The sensitivity of the Xpert assay for detection of M. tuberculosis in stool samples of PTB patients was 90.2% (95% CI, 82.9-95.0). All 50 stool samples from healthy individuals were negative by the assay (Specificity 100%; 95% CI, 92.9-100). Compared with the sputum culture positive results the sensitivity of the stool Xpert assay was 94.8% (95% CI, 88.5-97.8). Moreover, stool Xpert demonstrated full concordant results with the sputum culture for detection of rifampicin susceptibility. The cycle threshold values of rpoB probes obtained from Xpert assay correlated significantly with the bacilli load in the corresponding stool (Spearman correlation = -0.40, P < 0.01) and sputum (Spearman correlation = -0.77, P < 0.01) samples as determined by microscopy. Stool Xpert can be applied as a potential alternative of sputum testing for detection of M. tuberculosis and accurate determination of RIF susceptibility in adult PTB patients. The assay would be beneficial for rapid diagnosis of PTB for those adult patients who cannot expectorate sputum.