Metabolic Heat Stress Adaption in Transition Cows: Differences in Macronutrient Oxidation between Late-Gestating and Early-Lactating German Holstein Dairy Cows

• Published in: PloS one Vol. 10; no. 5; p. e0125264
• Main Authors: Lamp, Ole, Derno, Michael, Otten, Winfried, Mielenz, Manfred, Nürnberg, Gerd, Kuhla, Björn
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.05.2015; Public Library of Science (PLoS)
• Subjects: Adaptation, Physiological; Adipose tissue; Ambient temperature; Amino acids; Analysis; Animal Feed; Animal lactation; Animal sciences; Animals; Biological effects; Biology; Bovidae; Calorimetry; Carbohydrates; Care and treatment; Catabolism; Catecholamines - blood; Cattle; Complications and side effects; Creatinine; Dairy cattle; Energy Metabolism; Epinephrine; Esterification; Experiments; Farmers; Fatty acids; Feeding; Feeds; Female; Fetuses; Heat; Heat stress; Heat stress disorders; Heat tolerance; Heat-Shock Response; Hematocrit; Histidine; Hot Temperature; Humidity indexes; Influence; Lactation; Lactose; Metabolism; Metabolites; Metabolome; Methylhistidines - blood; Milk; Nitrogen; Noradrenaline; Norepinephrine; Nutrition; Nutritional requirements; Oxidation; Oxidation-Reduction; Oxidation-reduction reactions; Oxidative metabolism; Physiological aspects; Physiology; Plasma; Postpartum Period; Renal function; Temperature effects; Test chambers; Urea; Urine; Zoology; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0125264

High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS) and one pair-fed (PF) at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1), cows were challenged for 6 days (P2) by heat stress (temperature humidity index (THI) = 76) or thermoneutral pair-feeding in climatic chambers 3 weeks ante partum and again 3 weeks post-partum. On the sixth day of each period P1 or P2, oxidative metabolism was analyzed for 24 hours in open circuit respiration chambers. Water and feed intake, vital parameters and milk yield were recorded. Daily blood samples were analyzed for glucose, β-hydroxybutyric acid, non-esterified fatty acids, urea, creatinine, methyl histidine, adrenaline and noradrenaline. In general, heat stress caused marked effects on water homeorhesis with impairments of renal function and a strong adrenergic response accompanied with a prevalence of carbohydrate oxidation over fat catabolism. Heat-stressed cows extensively degraded tissue protein as reflected by the increase of plasma urea, creatinine and methyl histidine concentrations. However, the acute metabolic heat stress response in dry cows differed from early-lactating cows as the prepartal adipose tissue was not refractory to lipolytic, adrenergic stimuli, and the rate of amino acid oxidation was lower than in the postpartal stage. Together with the lower endogenous metabolic heat load, metabolic adaption in dry cows is indicative for a higher heat tolerance and the prioritization of the nutritional requirements of the fast-growing near-term fetus. These findings indicate that the development of future nutritional strategies for attenuating impairments of health and performance due to ambient heat requires the consideration of the physiological stage of dairy cows.