Changes in the Bacterial Microbiota in Gut, Blood, and Lungs following Acute LPS Instillation into Mice Lungs

• Published in: PloS one Vol. 9; no. 10; p. e111228
• Main Authors: Sze, Marc A., Tsuruta, Masashi, Yang, Shun-Wei Julia, Oh, Yeni, Man, S. F. Paul, Hogg, James C., Sin, Don D.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.10.2014; Public Library of Science (PLoS)
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - microbiology; Alveoli; Animals; Antibacterial agents; Antibiotics; Bacteria; Biology and Life Sciences; Blood; Bronchoalveolar Lavage Fluid - microbiology; Bronchus; Cecum; Cecum - microbiology; Chronic obstructive pulmonary disease; Deoxyribonucleic acid; Digestive system; Digestive tract; DNA; Gastrointestinal tract; Heart; Inflammation; Influenza; Laboratories; Lipopolysaccharides; Lipopolysaccharides - toxicity; Lung - drug effects; Lung - microbiology; Lungs; Medical research; Medicine; Medicine and Health Sciences; Mice; Microbiota; Microbiota (Symbiotic organisms); Microbiota - drug effects; Neomycin; Neomycin - pharmacology; Phyllobacteriaceae - drug effects; Phyllobacteriaceae - growth & development; Phyllobacteriaceae - isolation & purification; Polymerase chain reaction; Rodents; Sepsis; Serum - microbiology; Streptomycin; Streptomycin - pharmacology; Canada
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0111228

Previous reports have shown that the gastrointestinal (GI) bacterial microbiota can have profound effects on the lungs, which has been described as the "gut-lung axis". However, whether a "lung-gut" axis exists wherein acute lung inflammation perturbs the gut and blood microbiota is unknown. Adult C57/Bl6 mice were exposed to one dose of LPS or PBS instillation (n=3 for each group) directly into lungs. Bacterial microbiota of the bronchoalveolar lavage fluid, blood, and cecum were determined using 454 pyrotag sequencing and quantitative polymerase chain reaction (qPCR) at 4 through 168 hours post-instillation. We then investigated the effects of oral neomycin and streptomycin (n=8) on the microbiota at 4 and 24 hours post LPS instillation versus control treatment (n=5 at baseline and 4 hours, n=7 at 24 hours). At 24 hours post LPS instillation, the total bacterial count was significantly increased in the cecum (P<0.05); whereas the total bacterial count in blood was increased at 4, 48, and 72 hours (P<0.05). Antibiotic treatment reduced the total bacteria in blood but not in the cecum. The increase in total bacteria in the blood correlated with Phyllobacteriaceae OTU 40 and was significantly reduced in the blood for both antibiotic groups (P<0.05). LPS instillation in lungs leads to acute changes in the bacterial microbiota in the blood and cecum, which can be modulated with antibiotics.