Individuals Maintain Similar Rates of Protein Synthesis over Time on the Same Plane of Nutrition under Controlled Environmental Conditions

• Published in: PloS one Vol. 11; no. 3; p. e0152239
• Main Authors: McCarthy, Ian D, Owen, Stewart F, Watt, Peter W, Houlihan, Dominic F
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.03.2016; Public Library of Science (PLoS)
• Subjects: Aging (natural); Amino acids; Analysis; Animals; Biology and Life Sciences; Breakdowns; Controlled conditions; Ecophysiology; Environmental changes; Environmental conditions; Female; Fish; Fish Proteins - biosynthesis; Fitness; Flounder; Flounder - growth & development; Flounder - metabolism; Health aspects; Isotopes; Male; Meals; Medicine and Health Sciences; Metabolism; Natural selection; Nitrogen; Nitrogen Isotopes - chemistry; Nitrogen Isotopes - metabolism; Nutrition; Nutrition Assessment; Ontogeny; Physical fitness; Physical Sciences; Physiology; Platichthys flesus; Protein biosynthesis; Protein metabolism; Protein synthesis; Protein turnover; Proteins; Quantitative genetics; Reproducibility; Research and Analysis Methods; Seasonal variations; Stochasticity; Studies; United Kingdom > UK; New York; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0152239

Consistent individual differences in animal performance drive individual fitness under variable environmental conditions and provide the framework through which natural selection can operate. Underlying this concept is the assumption that individuals will display consistent levels of performance in fitness-related traits and interest has focused on individual variation and broad sense repeatability in a range of behavioural and physiological traits. Despite playing a central role in maintenance and growth, and with considerable inter-individual variation documented, broad sense repeatability in rates of protein synthesis has not been assessed. In this study we show for the first time that juvenile flounder Platichthys flesus reared under controlled environmental conditions on the same plane of nutrition for 46 days maintain consistent whole-animal absolute rates of protein synthesis (As). By feeding meals containing 15N-labelled protein and using a stochastic end-point model, two non-terminal measures of protein synthesis were made 32 days apart (d14 and d46). As values (mass-corrected to a standard mass of 12 g) showed 2- to 3-fold variation between individuals on d14 and d46 but individuals showed similar As values on both days with a broad sense repeatability estimate of 0.684 indicating significant consistency in physiological performance under controlled experimental conditions. The use of non-terminal methodologies in studies of animal ecophysiology to make repeat measures of physiological performance enables known individuals to be tracked across changing conditions. Adopting this approach, repeat measures of protein synthesis under controlled conditions will allow individual ontogenetic changes in protein metabolism to be assessed to better understand the ageing process and to determine individual physiological adaptive capacity, and associated energetic costs of adaptation, to global environmental change.