New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood

Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector,...

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Published inPloS one Vol. 9; no. 12; p. e113052
Main Authors Fujie, Yasumitsu, Fusaki, Noemi, Katayama, Tomohiko, Hamasaki, Makoto, Soejima, Yumi, Soga, Minami, Ban, Hiroshi, Hasegawa, Mamoru, Yamashita, Satoshi, Kimura, Shigemi, Suzuki, Saori, Matsuzawa, Tetsuro, Akari, Hirofumi, Era, Takumi
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 05.12.2014
Public Library of Science (PLoS)
Subjects
Analysis
Animals
Biology and Life Sciences
Blood
Blood cells
Cell culture
Cell Differentiation - genetics
Cell Line
Cellular Reprogramming - genetics
Deoxyribonucleic acid
Disease
DNA
DNA microarrays
Embryology
Embryonic stem cells
Evolution
Fibroblasts
Gene Expression
Genetic Vectors
Genomes
Health aspects
Humans
Immunology
Induced Pluripotent Stem Cells - cytology
KLF4 protein
Mammals
Medicine and Health Sciences
Monkeys & apes
Mutation
Oct-4 protein
Pan troglodytes
Physiology
Plasmids
Pluripotency
RNA polymerase
Science
Sendai virus - genetics
Stem cells
Temperature
Therapeutic applications
Transduction, Genetic
Transgenes
Viruses
Japan
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Summary:Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.
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Conceived and designed the experiments: TE. Performed the experiments: YF TK M. Hamasaki YS MS NF. Analyzed the data: YF TK M. Hamasaki YS MS NF. Contributed reagents/materials/analysis tools: NF HB M. Hasegawa SY SK SS TM HA. Wrote the paper: TE.
Competing Interests: HB is an employee of DNAVEC Corporation. M. Hasegawa is a founder and adviser of DNAVEC Corporation. NF was an employee of DNAVEC Corporation until January 2013 but not now. The commercial product developed by DNAVEC Corporation is similar to the vectors described in this paper but the component is different. The patent of the Sendai virus vectors to generate iPS cells that was applied by and of DNAVEC Corporation is pending (WO/2010/008054). NF and HB have waived the right of the patent. These do not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
Current address: Department of Ophthalmology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582, Japan
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0113052