Nuclear-cytoplasmic conflict in pea (Pisum sativum L.) is associated with nuclear and plastidic candidate genes encoding acetyl-CoA carboxylase subunits

• Published in: PloS one Vol. 10; no. 3; p. e0119835
• Main Authors: Bogdanova, Vera S, Zaytseva, Olga O, Mglinets, Anatoliy V, Shatskaya, Natalia V, Kosterin, Oleg E, Vasiliev, Gennadiy V
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.03.2015; Public Library of Science (PLoS)
• Subjects: Acetyl-CoA carboxylase; Acetyl-CoA Carboxylase - genetics; Alfalfa; Amino acids; Biopolymers; Biotin; Biotin carboxyl carrier protein; Carboxyltransferase; Cell Nucleolus - genetics; Cellular biology; Chloroplasts; Compatibility; Cytoplasm; Cytoplasm - genetics; Cytoplasmic incompatibility; Deoxyribonucleic acid; DNA; Evolution; Fertility; Gene expression; Gene sequencing; Genes; Genetic aspects; Genetic engineering; Genome, Plant; Genomes; Genomics; Harbors; High-Throughput Nucleotide Sequencing; Hybridization; Incompatibility; Lethality; Loci; Molecular Sequence Data; Next-generation sequencing; Pea; Peas; Pisum sativum; Pisum sativum - genetics; Plastids - genetics; Pollen; Polypeptides; Proteins; Synteny; Israel; Russia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0119835

In crosses of wild and cultivated peas (Pisum sativum L.), nuclear-cytoplasmic incompatibility frequently occurs manifested as decreased pollen fertility, male gametophyte lethality, sporophyte lethality. High-throughput sequencing of plastid genomes of one cultivated and four wild pea accessions differing in cross-compatibility was performed. Candidate genes for involvement in the nuclear-plastid conflict were searched in the reconstructed plastid genomes. In the annotated Medicago truncatula genome, nuclear candidate genes were searched in the portion syntenic to the pea chromosome region known to harbor a locus involved in the conflict. In the plastid genomes, a substantial variability of the accD locus represented by nucleotide substitutions and indels was found to correspond to the pattern of cross-compatibility among the accessions analyzed. Amino acid substitutions in the polypeptides encoded by the alleles of a nuclear locus, designated as Bccp3, with a complementary function to accD, fitted the compatibility pattern. The accD locus in the plastid genome encoding beta subunit of the carboxyltransferase of acetyl-coA carboxylase and the nuclear locus Bccp3 encoding biotin carboxyl carrier protein of the same multi-subunit enzyme were nominated as candidate genes for main contribution to nuclear-cytoplasmic incompatibility in peas. Existence of another nuclear locus involved in the accD-mediated conflict is hypothesized.