Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro

• Published in: PloS one Vol. 14; no. 9; p. e0222240
• Main Authors: Modi, Prashant Kumar, Prabhu, Ashwini, Bhandary, Yashodhar P, Shenoy P, Sudheer, Hegde, Aparna, Es, Sindhu Priya, Johnson, Renjith P, Das, Shankar Prasad, Vazirally, Sahil, Rekha, Punchappady-Devasya
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.09.2019; Public Library of Science (PLoS)
• Subjects: Acids; Alizarin; Apoptosis; Bioavailability; Biological effects; Biology and Life Sciences; Biomedical materials; Bone remodeling; Bone resorption; Caco-2 Cells; Calcium; Calcium - metabolism; Calcium influx; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Cell viability; Cells, Cultured; Collagen; Collagen Type I - metabolism; Cysteine; Cystine; Cytotoxicity; EDTA; Epithelial cells; Fluorescent antibody technique; Gene expression; Genes; Health aspects; HEK293 Cells; Humans; Immunofluorescence; Medicine and Health Sciences; Mineralization; Muscle contraction; Muscles; Muscular function; Neurotransmission; Nutrient deficiency; Organic chemistry; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteocalcin; Osteocalcin - metabolism; Osteogenesis; Osteogenesis - drug effects; Osteonectin; Osteonectin - metabolism; Osteopontin; Osteopontin - metabolism; Osteoporosis; Pharmaceuticals; Research and Analysis Methods; Signal transduction; Skeletal muscle; Stem cells; Structure-function relationships; Sugar Acids - pharmacology; Supplements; Toxicity; Vitamin D; Water chemistry; Western blotting; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0222240

Calcium is the key macromineral having a role in skeletal structure and function, muscle contraction, and neurotransmission. Bone remodeling is maintained through a constant balance between calcium resorption and deposition. Calcium deficiency is resolved through calcium supplementation, and among the supplements, water-soluble organic molecules attracted great pharmaceutical interest. Calcium glucoheptonate is a highly water-soluble organic calcium salt having clinical use; however, detailed investigations on its biological effects are limited. We assessed the effects of calcium glucoheptonate on cell viability and proliferation of osteoblast-like MG-63 cells. Calcium uptake and mineralization were evaluated using Alizarin red staining of osteoblast-like MG-63 cells treated with calcium glucoheptonate. Expression of osteogenic markers were monitored by western blotting, immunofluorescence, and qRT-PCR assays. Increased proliferation and calcium uptake were observed in the MG-63 cells treated with calcium glucoheptonate. The treatment also increased the expression of osteopontin and osteogenic genes such as collagen-1, secreted protein acidic and cysteine rich (SPARC), and osteocalcin. Calcium glucoheptonate treatment did not exert any cytotoxicity on colorectal and renal epithelial cells, indicating the safety of the treatment. This is the first report with evidence for its beneficial effect for pharmaceutical use in addressing calcium deficiency conditions.