RNA-Seq and Microarrays Analyses Reveal Global Differential Transcriptomes of Mesorhizobium huakuii 7653R between Bacteroids and Free-Living Cells

• Published in: PloS one Vol. 9; no. 4; p. e93626
• Main Authors: Peng, Jieli, Hao, Baohai, Liu, Liu, Wang, Shanming, Ma, Binguang, Yang, Yi, Xie, Fuli, Li, Youguo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.04.2014; Public Library of Science (PLoS)
• Subjects: Analysis; Astragalus sinicus; Bacteria; Bacteroids; Bioinformatics; Biological effects; Biology and Life Sciences; Bradyrhizobium japonicum; Cell cycle; Cell division; Cells (biology); Developmental stages; DNA microarrays; Energy metabolism; Fatty acids; Gene expression; Genes; Genes, Bacterial; Genomes; Genomics; Host plants; Kinases; Laboratories; Legumes; Life sciences; Mesorhizobium - genetics; Mesorhizobium huakuii; Metabolism; Methods; Nitrogen; Nitrogen fixation; Oligonucleotide Array Sequence Analysis; Plasmids; Protein interaction; Proteins; Research and Analysis Methods; Ribonucleic acid; RNA; Sequence Analysis, RNA; Signal transduction; Sinorhizobium meliloti; Studies; Symbiosis; Transcriptome
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0093626

Mesorhizobium huakuii 7653R occurs either in nitrogen-fixing symbiosis with its host plant, Astragalus sinicus, or free-living in the soil. The M. huakuii 7653R genome has recently been sequenced. To better understand the complex biochemical and developmental changes that occur in 7653R during bacteroid development, RNA-Seq and Microarrays were used to investigate the differential transcriptomes of 7653R bacteroids and free-living cells. The two approaches identified several thousand differentially expressed genes. The most prominent up-regulation occurred in the symbiosis plasmids, meanwhile gene expression is concentrated to a set of genes (clusters) in bacteroids to fulfill corresponding functional requirements. The results suggested that the main energy metabolism is active while fatty acid metabolism is inactive in bacteroid and that most of genes relevant to cell cycle are down-regulated accordingly. For a global analysis, we reconstructed a protein-protein interaction (PPI) network for 7653R and integrated gene expression data into the network using Cytoscape. A highly inter-connected subnetwork, with function enrichment for nitrogen fixation, was found, and a set of hubs and previously uncharacterized genes participating in nitrogen fixation were identified. The results described here provide a broader biological landscape and novel insights that elucidate rhizobial bacteroid differentiation, nitrogen fixation and related novel gene functions.