Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE)...
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| Published in | PloS one Vol. 17; no. 1; p. e0262677 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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21.01.2022
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| Abstract | Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity. |
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| AbstractList | Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity. Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity.Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity. |
| Audience | Academic |
| Author | Kwiatkowska, Joanna Kalkowski, Lukasz Golubczyk, Dominika Walczak, Piotr Domzalska, Malgorzata Malysz-Cymborska, Izabela |
| AuthorAffiliation | 2 Center for Advanced Imaging Research and Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States of America Texas Biomedical Research Institute, UNITED STATES 1 Department of Neurosurgery, School of Medicine, Collegium Medicum, University of Warmia and Mazury, Olsztyn, Poland |
| AuthorAffiliation_xml | – name: Texas Biomedical Research Institute, UNITED STATES – name: 1 Department of Neurosurgery, School of Medicine, Collegium Medicum, University of Warmia and Mazury, Olsztyn, Poland – name: 2 Center for Advanced Imaging Research and Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States of America |
| Author_xml | – sequence: 1 givenname: Lukasz orcidid: 0000-0002-2445-9030 surname: Kalkowski fullname: Kalkowski, Lukasz – sequence: 2 givenname: Dominika surname: Golubczyk fullname: Golubczyk, Dominika – sequence: 3 givenname: Joanna surname: Kwiatkowska fullname: Kwiatkowska, Joanna – sequence: 4 givenname: Malgorzata surname: Domzalska fullname: Domzalska, Malgorzata – sequence: 5 givenname: Piotr surname: Walczak fullname: Walczak, Piotr – sequence: 6 givenname: Izabela surname: Malysz-Cymborska fullname: Malysz-Cymborska, Izabela |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35061807$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1007_s12017_025_08837_1 crossref_primary_10_1186_s13578_023_01079_3 crossref_primary_10_3390_nu14194119 |
| Cites_doi | 10.1124/jpet.107.127183 10.1016/S1474-4422(18)30443-5 10.2353/ajpath.2010.090437 10.1016/j.jns.2009.04.025 10.1073/pnas.1912012117 10.1016/S1474-4422(13)70102-9 10.1093/jnen/61.6.539 10.1084/jem.58.1.39 10.1371/journal.pone.0204650 10.1161/hs1101.097397 10.1016/j.ncl.2004.09.002 10.2353/ajpath.2008.070918 10.1038/s41598-021-85820-3 10.1016/j.pathophys.2010.04.004 10.1007/s40120-019-0146-z 10.1096/fj.11-183772 10.1007/s12035-019-1488-3 10.3791/51275 10.1016/S0091-3057(02)01053-5 10.5213/inj.2040156.078 10.1016/S0002-9440(10)63232-4 10.1002/glia.21026 10.1111/j.1365-2249.2004.02418.x 10.1038/s41598-020-74411-3 10.1016/j.expneurol.2013.09.009 |
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| Copyright | COPYRIGHT 2022 Public Library of Science 2022 Kalkowski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2022 Kalkowski et al 2022 Kalkowski et al |
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| References | R Allavena (pone.0262677.ref031) 2010; 176 D Golubczyk (pone.0262677.ref009) 2019; 56 L Kalkowski (pone.0262677.ref010) 2018; 13 AH All (pone.0262677.ref008) 2009; 284 CF Lucchinetti (pone.0262677.ref024) 2005; 23 EE McCandless (pone.0262677.ref025) 2008; 172 G Hashim (pone.0262677.ref015) 1986 JW Peterson (pone.0262677.ref030) 2002; 61 S Bittner (pone.0262677.ref019) 2014 K Selmaj (pone.0262677.ref002) 2013; 12 I Wimmer (pone.0262677.ref027) 2019; 10 A Ghezzi (pone.0262677.ref003) 2019; 8 H Karhunen (pone.0262677.ref007) 2003; 74 A Denic (pone.0262677.ref005) 2011; 18 A Ghaffarinia (pone.0262677.ref018) 2015; 10 MT Wallin (pone.0262677.ref001) 2019; 18 J-Y Kim (pone.0262677.ref020) 2020; 24 S Serres (pone.0262677.ref029) 2011; 25 S Stosic-Grujicic (pone.0262677.ref006) 2004; 136 CA Foster (pone.0262677.ref016) 2007; 323 D Golubczyk (pone.0262677.ref011) 2020; 10 BFG Popescu (pone.0262677.ref023) 2013; 19 I Malysz-Cymborska (pone.0262677.ref012) 2021; 11 L Zhang (pone.0262677.ref017) 2018; 16 M Kerschensteiner (pone.0262677.ref013) 2004; 164 M Sasaki (pone.0262677.ref014) 2010; 58 RL Roof (pone.0262677.ref021) 2001; 32 OA Marcos-Contreras (pone.0262677.ref028) 2020; 117 R-J Su (pone.0262677.ref022) 2018; 17 TM Rivers (pone.0262677.ref004) 1933; 58 A Boroujerdi (pone.0262677.ref026) 2013; 250 |
| References_xml | – volume: 323 start-page: 469 issue: 2 year: 2007 ident: pone.0262677.ref016 article-title: Brain Penetration of the Oral Immunomodulatory Drug FTY720 and Its Phosphorylation in the Central Nervous System during Experimental Autoimmune Encephalomyelitis: Consequences for Mode of Action in Multiple Sclerosis publication-title: Journal of Pharmacology and Experimental Therapeutics doi: 10.1124/jpet.107.127183 – volume: 10 issue: 711 year: 2019 ident: pone.0262677.ref027 article-title: PECAM-1 Stabilizes Blood-Brain Barrier Integrity and Favors Paracellular T-Cell Diapedesis Across the Blood-Brain Barrier During Neuroinflammation publication-title: Frontiers in Immunology – volume: 18 start-page: 269 issue: 3 year: 2019 ident: pone.0262677.ref001 article-title: Global, regional, and national burden of multiple sclerosis 1990–2016: a systematic analysis for the Global Burden of Disease Study 2016 publication-title: The Lancet Neurology doi: 10.1016/S1474-4422(18)30443-5 – volume: 176 start-page: 556 issue: 2 year: 2010 ident: pone.0262677.ref031 article-title: CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis publication-title: Am J Pathol doi: 10.2353/ajpath.2010.090437 – volume: 284 start-page: 81 issue: 1–2 year: 2009 ident: pone.0262677.ref008 article-title: Effect of MOG sensitization on somatosensory evoked potential in Lewis rats publication-title: Journal of the neurological sciences doi: 10.1016/j.jns.2009.04.025 – volume: 19 start-page: 901 issue: 4 year: 2013 ident: pone.0262677.ref023 article-title: Pathology of multiple sclerosis: where do we stand? publication-title: Continuum (Minneap Minn) – start-page: 16 year: 1986 ident: pone.0262677.ref015 article-title: Biological activity of region 65 102 of the myelin basic protein publication-title: Journal of Neuroscience Research – volume: 117 start-page: 3405 issue: 7 year: 2020 ident: pone.0262677.ref028 article-title: Selective targeting of nanomedicine to inflamed cerebral vasculature to enhance the blood-brain barrier publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.1912012117 – volume: 12 start-page: 756 issue: 8 year: 2013 ident: pone.0262677.ref002 article-title: Siponimod for patients with relapsing-remitting multiple sclerosis (BOLD): an adaptive, dose-ranging, randomised, phase 2 study publication-title: The Lancet Neurology doi: 10.1016/S1474-4422(13)70102-9 – volume: 61 start-page: 539 issue: 6 year: 2002 ident: pone.0262677.ref030 article-title: VCAM-1-Positive Microglia Target Oligodendrocytes at the Border of Multiple Sclerosis Lesions publication-title: Journal of Neuropathology & Experimental Neurology doi: 10.1093/jnen/61.6.539 – volume: 17 start-page: 3356 issue: 2 year: 2018 ident: pone.0262677.ref022 article-title: Time-course behavioral features are correlated with Parkinson’s disease-associated pathology in a 6-hydroxydopamine hemiparkinsonian rat model publication-title: Mol Med Rep – volume: 16 start-page: 4401 issue: 6 year: 2018 ident: pone.0262677.ref017 article-title: Activation of astrocytes and expression of inflammatory cytokines in rats with experimental autoimmune encephalomyelitis publication-title: Exp Ther Med – volume: 58 start-page: 39 issue: 1 year: 1933 ident: pone.0262677.ref004 article-title: OBSERVATIONS ON ATTEMPTS TO PRODUCE ACUTE DISSEMINATED ENCEPHALOMYELITIS IN MONKEYS publication-title: J Exp Med doi: 10.1084/jem.58.1.39 – volume: 13 start-page: e0204650 issue: 10 year: 2018 ident: pone.0262677.ref010 article-title: MRI-guided intracerebral convection-enhanced injection of gliotoxins to induce focal demyelination in swine publication-title: PLoS One doi: 10.1371/journal.pone.0204650 – volume: 32 start-page: 2648 issue: 11 year: 2001 ident: pone.0262677.ref021 article-title: A comparison of long-term functional outcome after 2 middle cerebral artery occlusion models in rats publication-title: Stroke doi: 10.1161/hs1101.097397 – volume: 23 start-page: 77 issue: 1 year: 2005 ident: pone.0262677.ref024 article-title: The pathology of multiple sclerosis publication-title: Neurol Clin doi: 10.1016/j.ncl.2004.09.002 – volume: 172 start-page: 799 issue: 3 year: 2008 ident: pone.0262677.ref025 article-title: Pathological expression of CXCL12 at the blood-brain barrier correlates with severity of multiple sclerosis publication-title: Am J Pathol doi: 10.2353/ajpath.2008.070918 – volume: 11 start-page: 6581 issue: 1 year: 2021 ident: pone.0262677.ref012 article-title: Intra-arterial transplantation of stem cells in large animals as a minimally-invasive strategy for the treatment of disseminated neurodegeneration publication-title: Sci Rep doi: 10.1038/s41598-021-85820-3 – volume: 18 start-page: 21 issue: 1 year: 2011 ident: pone.0262677.ref005 article-title: The relevance of animal models in multiple sclerosis research publication-title: Pathophysiology doi: 10.1016/j.pathophys.2010.04.004 – volume: 8 start-page: 461 issue: 2 year: 2019 ident: pone.0262677.ref003 article-title: Long-Term Effect of Immediate Versus Delayed Fingolimod Treatment in Young Adult Patients with Relapsing-Remitting Multiple Sclerosis: Pooled Analysis from the FREEDOMS/FREEDOMS II Trials publication-title: Neurol Ther doi: 10.1007/s40120-019-0146-z – volume: 25 start-page: 4415 issue: 12 year: 2011 ident: pone.0262677.ref029 article-title: VCAM-1-targeted magnetic resonance imaging reveals subclinical disease in a mouse model of multiple sclerosis publication-title: FASEB J doi: 10.1096/fj.11-183772 – volume: 56 start-page: 5740 issue: 8 year: 2019 ident: pone.0262677.ref009 article-title: The Role of Glia in Canine Degenerative Myelopathy: Relevance to Human Amyotrophic Lateral Sclerosis publication-title: Mol Neurobiol doi: 10.1007/s12035-019-1488-3 – issue: 86 year: 2014 ident: pone.0262677.ref019 article-title: Myelin oligodendrocyte glycoprotein (MOG35-55) induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice publication-title: J Vis Exp doi: 10.3791/51275 – volume: 74 start-page: 663 issue: 3 year: 2003 ident: pone.0262677.ref007 article-title: Forelimb use after focal cerebral ischemia in rats treated with an alpha 2-adrenoceptor antagonist publication-title: Pharmacology, biochemistry, and behavior doi: 10.1016/S0091-3057(02)01053-5 – volume: 24 start-page: S39 issue: Suppl 1 year: 2020 ident: pone.0262677.ref020 article-title: Swimming Exercise Ameliorates Symptoms of MOG-Induced Experimental Autoimmune Encephalomyelitis by Inhibiting Inflammation and Demyelination in Rats publication-title: Int Neurourol J doi: 10.5213/inj.2040156.078 – volume: 164 start-page: 1455 issue: 4 year: 2004 ident: pone.0262677.ref013 article-title: Targeting experimental autoimmune encephalomyelitis lesions to a predetermined axonal tract system allows for refined behavioral testing in an animal model of multiple sclerosis publication-title: Am J Pathol doi: 10.1016/S0002-9440(10)63232-4 – volume: 58 start-page: 1523 issue: 13 year: 2010 ident: pone.0262677.ref014 article-title: Focal experimental autoimmune encephalomyelitis in the lewis rat induced by immunization with myelin oligodendrocyte glycoprotein and intraspinal injection of vascular endothelial growth factor publication-title: Glia doi: 10.1002/glia.21026 – volume: 10 start-page: 9 issue: 1 year: 2015 ident: pone.0262677.ref018 article-title: Liver Damage and Mortality in a Male Lewis Rat of Experimental Autoimmune Encephalomyelitis publication-title: Iran J Pathol – volume: 136 start-page: 49 issue: 1 year: 2004 ident: pone.0262677.ref006 article-title: Induction of experimental autoimmune encephalomyelitis in Dark Agouti rats without adjuvant publication-title: Clinical and experimental immunology doi: 10.1111/j.1365-2249.2004.02418.x – volume: 10 start-page: 17318 issue: 1 year: 2020 ident: pone.0262677.ref011 article-title: Endovascular model of ischemic stroke in swine guided by real-time MRI publication-title: Sci Rep doi: 10.1038/s41598-020-74411-3 – volume: 250 start-page: 43 year: 2013 ident: pone.0262677.ref026 article-title: Extensive vascular remodeling in the spinal cord of pre-symptomatic experimental autoimmune encephalomyelitis mice; increased vessel expression of fibronectin and the α5β1 integrin publication-title: Exp Neurol doi: 10.1016/j.expneurol.2013.09.009 |
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| SubjectTerms | Analysis Animal models Animals Antibodies Antibodies - immunology Autoimmunity Biology and Life Sciences Blood-brain barrier Brain - pathology Brain research Care and treatment Cattle Demyelination Diagnosis Encephalomyelitis Encephalomyelitis, Autoimmune, Experimental - etiology Encephalomyelitis, Autoimmune, Experimental - pathology Epitopes Experimental allergic encephalomyelitis Growth factors Histology Immunization Injection Injections, Intraventricular Lesions Male Medicine Medicine and Health Sciences Morbidity Multiple sclerosis Myelin Neurological complications Neurosurgery Placement Rats Rats, Wistar Reproducibility Spinal cord Spinal Cord - immunology Vascular endothelial growth factor Video recorders |
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| Title | Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement |
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