Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement

Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE)...

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Published inPloS one Vol. 17; no. 1; p. e0262677
Main Authors Kalkowski, Lukasz, Golubczyk, Dominika, Kwiatkowska, Joanna, Domzalska, Malgorzata, Walczak, Piotr, Malysz-Cymborska, Izabela
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 21.01.2022
Public Library of Science (PLoS)
Subjects
Analysis
Animal models
Animals
Antibodies
Antibodies - immunology
Autoimmunity
Biology and Life Sciences
Blood-brain barrier
Brain - pathology
Brain research
Care and treatment
Cattle
Demyelination
Diagnosis
Encephalomyelitis
Encephalomyelitis, Autoimmune, Experimental - etiology
Encephalomyelitis, Autoimmune, Experimental - pathology
Epitopes
Experimental allergic encephalomyelitis
Growth factors
Histology
Immunization
Injection
Injections, Intraventricular
Lesions
Male
Medicine
Medicine and Health Sciences
Morbidity
Multiple sclerosis
Myelin
Neurological complications
Neurosurgery
Placement
Rats
Rats, Wistar
Reproducibility
Spinal cord
Spinal Cord - immunology
Vascular endothelial growth factor
Video recorders
Poland
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Summary:Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0262677