Cardiac function and architecture are maintained in a model of cardiorestricted overexpression of the prorenin-renin receptor

• Published in: PloS one Vol. 9; no. 2; p. e89929
• Main Authors: Mahmud, Hasan, Candido, Wellington Mardoqueu, van Genne, Linda, Vreeswijk-Baudoin, Inge, Yu, Hongjuan, van de Sluis, Bart, van Deursen, Jan, van Gilst, Wiek H, Silljé, Herman H W, de Boer, Rudolf A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.02.2014; Public Library of Science (PLoS)
• Subjects: Abnormalities; Acidification; Angiotensin; Angiotensins; Animals; Biology; Blood Pressure; Blotting, Western; Cardiology; Cardiomegaly - genetics; Cardiomegaly - metabolism; Cloning; Creatine Kinase - metabolism; Diabetes; Echocardiography; Experiments; Fibrosis; Gene expression; Gene Expression Regulation - genetics; Genetic engineering; Heart; Heart - physiology; Heart diseases; Heart failure; HeLa Cells; Humans; Hypertension; Hypertrophy; Hypoxia; Hypoxia - metabolism; Ischemia; Isoproterenol; Kidneys; Kinases; L-Lactate Dehydrogenase - metabolism; Laboratory animals; Major histocompatibility complex; Male; Medicine; Mice; Mice, Transgenic; Myocardium - metabolism; Protein folding; Real-Time Polymerase Chain Reaction; Receptors, Cell Surface - metabolism; Renin; Reperfusion; Reperfusion Injury - metabolism; RNA; Rodents; Smooth muscle; Transgenic animals; Transgenic mice; United States > US; Netherlands; Minnesota
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0089929

The (pro)renin-renin receptor, (P)RR has been claimed to be a novel element of the renin-angiotensin system (RAS). The function of (P)RR has been widely studied in renal and vascular pathology but the cardio-specific function of (P)RR has not been studied in detail. We therefore generated a transgenic mouse (Tg) with cardio-restricted (P)RR overexpression driven by the alpha-MHC promotor. The mRNA expression of (P)RR was ∼ 170-fold higher (P<0.001) and protein expression ∼ 5-fold higher (P<0.001) in hearts of Tg mice as compared to non-transgenic (wild type, Wt) littermates. This level of overexpression was not associated with spontaneous cardiac morphological or functional abnormalities in Tg mice. To assess whether (P)RR could play a role in cardiac hypertrophy, we infused ISO for 28 days, but this caused an equal degree of cardiac hypertrophy and fibrosis in Wt and Tg mice. In addition, ischemia-reperfusion injury was performed in Langendorff perfused isolated mouse hearts. We did not observe differences in parameters of cardiac function or damage between Wt and Tg mouse hearts under these conditions. Finally, we explored whether the hypoxia sensing response would be modulated by (P)RR using HeLa cells with and without (P)RR overexpression. We did not establish any effect of (P)RR on expression of genes associated with the hypoxic response. These results demonstrate that cardio-specific overexpression of (P)RR does not provoke phenotypical differences in the heart, and does not affect the hearts' response to stress and injury. It is concluded that increased myocardial (P)RR expression is unlikely to have a major role in pathological cardiac remodeling.