Magnolol additive improves growth performance of Linwu ducklings by modulating antioxidative status

• Published in: PloS one Vol. 16; no. 12; p. e0259896
• Main Authors: Lin, Qian, Liu, Yang, Peng, Simin, Liu, Chunjie, Lv, Tuo, Liao, Liping, Li, Yinghui, Wang, Yanzhou, Fan, Zhiyong, Wu, Weiguo, Zeng, Jianguo, Qiu, Huajiao, He, Xi, Dai, Qiuzhong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.12.2021; Public Library of Science (PLoS)
• Subjects: Aging; Animal sciences; Animals; Antibiotics; Antioxidants; Antioxidants - metabolism; Biology and Life Sciences; Biomarkers; Biomarkers - blood; Biphenyl Compounds - pharmacology; Body weight; Body weight gain; Catalase; Colistin; Deoxyguanosine; Diet; Ducks - blood; Ducks - growth & development; Ethanol; Feed additives; Feeds; Female; Food additives; Food science; Gene expression; Gene Expression Regulation - drug effects; Genetic aspects; Glutathione; Glutathione peroxidase; Homology; Ileum; Influence; Intestinal Mucosa - drug effects; Intestine; Juveniles; Lignans - pharmacology; Liver; Liver - drug effects; Liver - metabolism; Magnolia; Medicine and Health Sciences; Morphology; Mucosa; Nutrients; Oxidative stress; Peroxidase; Peroxides - metabolism; Phytochemicals; RNA, Messenger - genetics; RNA, Messenger - metabolism; Small intestine; Sulfates; Superoxide dismutase; Variance analysis; Villus; Zoology; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0259896

Magnolol is a bioactive polyphenolic compound commonly found in Magnolia officinalis . The aim of this study is to clarify the contribution of the magnolol additive on the growth performance of Linwu ducklings aging from 7 to 28 d, comparing to the effects of antibiotic additive (colistin sulphate). A total of 325, 7-d-old ducklings were assigned to 5 groups. Each group had 5 cages with 13 ducklings in each cage. The ducklings in different groups were fed with diets supplemented with 0, 100, 200 and 300 mg/kg magnolol additive (MA) (Control, MA100, MA200 and MA300) and 30 mg/kg colistin sulphate (CS30) for 3 weeks, respectively. Parameters regarding to the growth performance, intestinal mucosal morphology, serum biochemical indices, antioxidant and peroxide biomarkers and the expression levels of antioxidant-related genes were evaluated by one way ANOVA analysis. The results showed that 30 mg/kg colistin sulphate, 200 and 300 mg/kg magnolol additive improved the average final weight ( P = 0.045), average daily body weight gain ( P = 0.038) and feed/gain ratios ( P = 0.001) compared to the control group. 200 and 300 mg/kg magnolol additive significantly increased the villus height/crypt depth ratio of ileum, compared to the control and CS30 groups ( P = 0.001). Increased serum level of glucose ( P = 0.011) and total protein ( P = 0.006) were found in MA200 or MA300 group. In addition, comparing to the control and CS30 groups, MA200 or MA300 significantly increased the levels of superoxide dismutase ( P = 0.038), glutathione peroxidase ( P = 0.048) and reduced glutathione ( P = 0.039) in serum. Moreover, the serum and hepatic levels of 8-hydroxy-2’-deoxyguanosine ( P = 0.043 and 0.007, respectively) were lower in all MA groups compared to those of the control and CS30 group. The hepatic mRNA expression levels of superoxide dismutase-1 , catalase and nuclear factor erythroid-2-related factor 2/erythroid-derived CNC-homology factor were also increased significantly in MA200 and MA300 groups ( P < 0.05). Taken together, these data demonstrated that MA was an effective feed additive enhancing the growth performance of Linwu ducklings at 7 to 28 d by improving the antioxidant and intestinal mucosal status. It suggested that MA could be a potential ingredient to replace the colistin sulphate in diets.