Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice

• Published in: PloS one Vol. 14; no. 1; p. e0210670
• Main Authors: Yu, Jia-Tian, Liu, Ye, Dong, Ping, Cheng, Run-En, Ke, Shao-Xi, Chen, Kai-Qin, Wang, Jing-Jing, Shen, Zhong-Shan, Tang, Qiong-Yao, Zhang, Zhe
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24.01.2019; Public Library of Science (PLoS)
• Subjects: Anesthesiology; Animals; Biology and Life Sciences; Brain; Brain damage; Brain injury; Care and treatment; Cerebellum; Convulsions & seizures; Diencephalon; Disease Models, Animal; Enzymes; Epilepsy; Gene expression; Gene mutation; International markets; Kindling; Kindling, Neurologic - genetics; Kindling, Neurologic - metabolism; Laboratories; Male; Medicine and Health Sciences; Mice; Nervous system; Oxidative stress; Pathogenesis; Phosphatase; Proteins; Reductase; Research and Analysis Methods; Risk factors; Rodents; Seizing; Seizures; Seizures - genetics; Seizures - metabolism; Thioredoxin; Thioredoxin Reductase 1 - genetics; Thioredoxin Reductase 1 - metabolism; Thioredoxins - genetics; Thioredoxins - metabolism; Up-regulation; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0210670

Oxidative stress has been considered as one of pathogenesis of brain damage led by epilepsy. Reducing oxidative stress can ameliorate brain damage during seizures. However, expression levels of important antioxidative enzymes such as thioredoxin-1 (TRX1), thioredoxin-like 1 protein (TXNL1) and thioredoxin reductase 1 (TXNRD1) during seizures have not been investigated. In this study, we examined protein and mRNA expression levels of TRX1, TXNL1 and TXNRD1 in different brain regions in PTZ induced seizure model mice. We found that protein expression levels of TRX1, TXNL1 and TXNRD1 are simultaneously up-regulated by 2- or 3-fold in the cortex of both acute and chronic seizure model mice. But there is no unified expression pattern change of these enzymes in the hippocampus, cerebellum and diencephalon in the seizure model mice. Less extent up-regulation of mRNA expression of these enzymes were also observed in the cortex of seizure mice. These data suggest that antioxidative enzymes may provide a protective effect against oxidative stress in the cortex during seizures.