A Novel Peptide from Soybean Protein Isolate Significantly Enhances Resistance of the Organism under Oxidative Stress

• Published in: PloS one Vol. 11; no. 7; p. e0159938
• Main Authors: Ma, Heran, Liu, Rui, Zhao, Ziyuan, Zhang, Zhixian, Cao, Yue, Ma, Yudan, Guo, Yi, Xu, Li
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.07.2016; Public Library of Science (PLoS)
• Subjects: Acquired immune deficiency syndrome; AIDS; Amino acid sequence; Animals; Animals, Genetically Modified; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological effects; Biological Products - chemistry; Biological Products - metabolism; Biological Products - pharmacology; Biology and Life Sciences; Caenorhabditis elegans; Chromatography; Clinical medicine; Cosmetics; Dosidicus gigas; Drosophila melanogaster; Education; Engineering; Enzymes; Food; Free radicals; Gene expression; Gene Expression Regulation; Glycine max; Health aspects; Health care; Hydrogen-Ion Concentration; Hydrolysates; Hydrolysis; Influence; Insects; Laboratories; Life sciences; Nutrition; Occupational health; Oxidation resistance; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Peptides; Peptides - chemistry; Peptides - isolation & purification; Peptides - pharmacology; Physical Sciences; Physiological aspects; Proteins; Reactive Oxygen Species; Research and Analysis Methods; Skin; Soybean Proteins - chemistry; Soybean Proteins - metabolism; Soybeans; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Tandem Mass Spectrometry
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0159938

Recent studies have indicated that protein hydrolysates have broad biological effects. In the current study we describe a novel antioxidative peptide, FDPAL, from soybean protein isolate (SPI). The aim of this study was to purify and characterize an antioxidative peptide from SPI and determine its antioxidative mechanism. LC-MS/MS was used to isolate and identify the peptide from SPI. The sequence of the peptide was determined to be Phe-Asp-Pro-Ala-Leu (FDPAL, 561 Da). FDPAL can cause significant enhancement of resistance to oxidative stress both in cells as well as simple organisms. In Caenorhabditis elegans (C. elegans), FDPAL can up-regulate the expression of certain genes associated with resistance. The antioxidant activity of this peptide can be attributed to the presence of a specific amino acid sequence. Results from our work suggest that FDPAL can facilitate potential applications of proteins carrying this sequence in the nutraceutical, bioactive material and clinical medicine areas, as well as in cosmetics and health care products.