A user-friendly platform for yeast two-hybrid library screening using next generation sequencing

• Published in: PloS one Vol. 13; no. 12; p. e0201270
• Main Authors: Erffelinck, Marie-Laure, Ribeiro, Bianca, Perassolo, Maria, Pauwels, Laurens, Pollier, Jacob, Storme, Veronique, Goossens, Alain
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.12.2018; Public Library of Science (PLoS)
• Subjects: Adaptation; Adaptations; Analysis; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Bioinformatics; Biology; Biology and life sciences; Biotechnology; Data processing; Deoxyribonucleic acid; DNA; DNA methylation; DNA sequencing; Gene Library; Genetics; Genomes; High-Throughput Nucleotide Sequencing; Identification; Jasmonic acid; Medical screening; Next-generation sequencing; Open Reading Frames; Plant sciences; Principal components analysis; Protein interaction; Protein-protein interactions; Proteins; Research and analysis methods; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Screening; Sequences; Technology; Transcription factors; Trends; Two-Hybrid System Techniques; Yeast; Belgium; Argentina
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0201270

Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish medium- to high-throughput Y2H screening pla...