A user-friendly platform for yeast two-hybrid library screening using next generation sequencing

• Published in: PloS one Vol. 13; no. 12; p. e0201270
• Main Authors: Erffelinck, Marie-Laure, Ribeiro, Bianca, Perassolo, Maria, Pauwels, Laurens, Pollier, Jacob, Storme, Veronique, Goossens, Alain
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.12.2018; Public Library of Science (PLoS)
• Subjects: Adaptation; Adaptations; Analysis; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Bioinformatics; Biology; Biology and life sciences; Biotechnology; Data processing; Deoxyribonucleic acid; DNA; DNA methylation; DNA sequencing; Gene Library; Genetics; Genomes; High-Throughput Nucleotide Sequencing; Identification; Jasmonic acid; Medical screening; Next-generation sequencing; Open Reading Frames; Plant sciences; Principal components analysis; Protein interaction; Protein-protein interactions; Proteins; Research and analysis methods; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Screening; Sequences; Technology; Transcription factors; Trends; Two-Hybrid System Techniques; Yeast; Belgium; Argentina
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0201270

Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish medium- to high-throughput Y2H screening platforms, with several having successfully incorporated the use of the next-generation sequencing (NGS) technology to increase the scale and sensitivity of the method. However, these have been to date mainly restricted to the use of fully annotated custom-made open reading frame (ORF) libraries and subject to complex downstream data processing. Here, a streamlined Y2H library screening strategy, based on integration of Y2H with NGS, called Y2H-seq, was developed, which allows efficient and reliable screening of Y2H cDNA libraries. To generate proof of concept, the method was applied to screen for interaction partners of two key components of the jasmonate signaling machinery in the model plant Arabidopsis thaliana, resulting in the identification of several previously reported as well as hitherto unknown interactors. Our Y2H-seq method offers a user-friendly, specific and sensitive screening method that allows identification of PPIs without prior knowledge of the organism's ORFs, thereby extending the method to organisms of which the genome has not entirely been annotated yet. The quantitative NGS readout allows to increase genome coverage, thereby overcoming some of the bottlenecks of current Y2H technologies, which will further strengthen the value of the Y2H technology as a discovery platform.