Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model

Mitochondrial electron transport chain (ETC) defects are observed in Parkinson's disease (PD) patients and in PD fly- and mouse-models; however it remains to be tested if acute improvement of ETC function alleviates PD-relevant defects. We tested the hypothesis that 808 nm infrared light that e...

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Published inPloS one Vol. 8; no. 11; p. e78562
Main Authors Vos, Melissa, Lovisa, Blaise, Geens, Ann, Morais, Vanessa A, Wagnières, Georges, van den Bergh, Hubert, Ginggen, Alec, De Strooper, Bart, Tardy, Yanik, Verstreken, Patrik
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 11.11.2013
Public Library of Science (PLoS)
Subjects
Adenosine Triphosphate - genetics
Adenosine Triphosphate - metabolism
Animal models
Animals
Biology
Defects
Disease
Disease Models, Animal
Dopamine
Dopamine receptors
Drosophila
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Electron transport
Electron transport chain
Electron Transport Complex IV - genetics
Electron Transport Complex IV - metabolism
Genetics
Humans
I.R. radiation
Infrared radiation
Insects
Lasers
Light
Membrane potential
Mice
Mitochondria
Mitochondrial DNA
Morphology
Movement disorders
Mutants
Neurodegenerative diseases
Neurosciences
Oxygen
Oxygen Consumption
Parkinson Disease - genetics
Parkinson Disease - metabolism
Parkinson's disease
Photonics
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
PTEN-induced putative kinase
Tissues
Wound healing
Belgium
United States > US
Switzerland
Germany
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Summary:Mitochondrial electron transport chain (ETC) defects are observed in Parkinson's disease (PD) patients and in PD fly- and mouse-models; however it remains to be tested if acute improvement of ETC function alleviates PD-relevant defects. We tested the hypothesis that 808 nm infrared light that effectively penetrates tissues rescues pink1 mutants. We show that irradiating isolated fly or mouse mitochondria with 808 nm light that is absorbed by ETC-Complex IV acutely improves Complex IV-dependent oxygen consumption and ATP production, a feature that is wavelength-specific. Irradiating Drosophila pink1 mutants using a single dose of 808 nm light results in a rescue of major systemic and mitochondrial defects. Time-course experiments indicate mitochondrial membrane potential defects are rescued prior to mitochondrial morphological defects, also in dopaminergic neurons, suggesting mitochondrial functional defects precede mitochondrial swelling. Thus, our data indicate that improvement of mitochondrial function using infrared light stimulation is a viable strategy to alleviate pink1-related defects.
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Conceived and designed the experiments: MV BL VAM YT PV A. Ginggen. Performed the experiments: MV A. Geens. Analyzed the data: MV MV BL VAL GW HvdB BDS YT PV. Wrote the paper: MV PV.
Competing Interests: The authors have read the journal’s policy and have the following conflicts: Bart De Strooper’s lab and Patrik Verstreken’s lab receive research funding from Janssen Pharmaceutica, Beerse, Belgium. Bart De Strooper is a consultant for Janssen Pharmaceutica, Envivo, and Remynd, Leuven, Belgium. Blaise Lovisa, Alec Gingen and Yanik Tardy are employees of Johnson&Johnson. However, the authors declare that this does not alter their adherence to all the PLOS ONE policies on sharing data and materials reported in this work.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0078562