Isolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya

• Published in: PloS one Vol. 14; no. 4; p. e0215734
• Main Authors: Akhwale, Juliah Khayeli, Rohde, Manfred, Rohde, Christine, Bunk, Boyke, Spröer, Cathrin, Boga, Hamadi Iddi, Klenk, Hans-Peter, Wittmann, Johannes
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.04.2019; Public Library of Science (PLoS)
• Subjects: Analysis; Bacteria; Bacteria - virology; Bacteriophages; Bacteriophages - genetics; Bacteriophages - isolation & purification; Bacteriophages - ultrastructure; Biodiversity; Cell culture; Chemical properties; Deoxyribonucleic acid; Disease transmission; DNA; E coli; Ecosystems; Electron microscopy; Environmental aspects; Extreme Environments; Gene polymorphism; Gene sequencing; Genome, Viral; Genomes; Genomics; Geologic Sediments - microbiology; Hydrogen-Ion Concentration; Identification and classification; Infection; Kenya; Lake sediments; Lakes; Lakes - chemistry; Lakes - microbiology; Microorganisms; Microscopy; Microscopy, Electron, Transmission; Open reading frames; Phages; Phylogeny; Polymorphism; Proteins; Restriction fragment length polymorphism; Salinity; Sediment samplers; Sediments (Geology); Transmission electron microscopy; Viral infections; Viruses; Water; Kenya; Germany

As a step towards better understanding of diversity and biology of phages and their hosts in haloalkaline Lake Elmenteita, phages were isolated from sediment samples and overlying water using indigenous bacteria as hosts. 17 seemingly different phages of diverse morphotypes with different dimensions...