Assessing the effect of starch digestion characteristics on ileal brake activation in broiler chickens

• Published in: PloS one Vol. 15; no. 2; p. e0228647
• Main Authors: Herwig, Eugenia, Schwean-Lardner, Karen, Van Kessel, Andrew, Savary, Rachel K, Classen, Henry L
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.02.2020; Public Library of Science (PLoS)
• Subjects: Activation; Analysis; Animal Nutritional Physiological Phenomena; Animals; Biology and Life Sciences; Blood levels; Body Weight; Brakes; Cell activation; Chickens; Chickens - physiology; Colon; Crop; Crops; Diet; Digestibility; Digestion; Digestive tract; Enzyme-linked immunosorbent assay; Experiments; Fatty acids; Fatty Acids, Volatile - metabolism; Feeds; Gastrointestinal tract; Gene expression; Genes; Glucagon; Glucagon-like peptide 1; Glucagon-Like Peptide 1 - blood; Ileum; Ileum - metabolism; Intestine; Jejunum; Levels; Light; Male; Medicine and Health Sciences; Morphology; Pea; Pens; Peptide YY - blood; Peptides; pH effects; Phenols (Class of compounds); Physical Sciences; Poultry; Proteins; Regression analysis; Small intestine; Starch; Starch - metabolism; Transcription; Tyrosine; Canada; Saskatchewan; Saskatchewan Canada
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0228647

The objective of this research was to evaluate activation of the ileal brake in broiler chickens using diets containing semi-purified wheat (WS; rapidly and highly digested) and pea (PS; slowly and poorly digested) starch. Diets were formulated to contain six WS:PS ratios (100:0, 80:20, 60:40, 40:60, 20:80, 0:100) and each starch ratio was fed to 236 Ross 308 male broilers housed in 4 litter floor pens. At 28 d of age, the effect of PS concentration was assessed on starch digestion, digestive tract morphology, and digesta pH and short-chain fatty acid (SCFA) concentration. Glucagon-like peptide-1 (GLP-1) and peptide tyrosine-tyrosine (PYY) status were assessed in serum (ELISA) and via gene expression in jejunal and ileal tissue (proglucagon for GLP-1). Data were analyzed using regression analyses, and significance was accepted at P ≤ 0.05. Increasing dietary PS resulted in reduced starch digestibility in the small intestine, but had no effect in the colon. Crop content pH responded quadratically to PS level with an estimated minimum at 55% PS. Total SCFA increased linearly in the crop with PS level, but changed in a quadratic fashion in the ileum (estimated maximum at 62% PS). Ceacal SCFA concentrations were highest for the 80 and 100% PS levels. The relative empty weight (crop, small intestine, colon), length (small intestine) and content (crop jejunum, Ileum) of digestive tract sections increased linearly with increasing PS concentration. Dietary treatment did not affect serum GLP-1 or PYY or small intestine transcript abundance. In conclusion, feeding PS increased the presence of L-cell activators (starch, SCFA) and increased trophic development and content of the digestive tract, suggestive of L-cell activation. However, no direct evidence of ileal brake activation was found by measuring venous blood levels of GLP-1 or PYY or corresponding gene expression in small intestine tissue.