Effect of broccoli sprouts on nasal response to live attenuated influenza virus in smokers: a randomized, double-blind study

Smokers have increased susceptibility and altered innate host defense responses to influenza virus infection. Broccoli sprouts are a source of the Nrf2 activating agentsulforaphane, and short term ingestion of broccoli sprout homogenates (BSH) has been shown to reduce nasal inflammatory responses to...

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Published inPloS one Vol. 9; no. 6; p. e98671
Main Authors Noah, Terry L, Zhang, Hongtao, Zhou, Haibo, Glista-Baker, Ellen, Müller, Loretta, Bauer, Rebecca N, Meyer, Megan, Murphy, Paula C, Jones, Shannon, Letang, Blanche, Robinette, Carole, Jaspers, Ilona
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 09.06.2014
Public Library of Science (PLoS)
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Summary:Smokers have increased susceptibility and altered innate host defense responses to influenza virus infection. Broccoli sprouts are a source of the Nrf2 activating agentsulforaphane, and short term ingestion of broccoli sprout homogenates (BSH) has been shown to reduce nasal inflammatory responses to oxidant pollutants. Assess the effects of BSH on nasal cytokines, virus replication, and Nrf2-dependent enzyme expression in smokers and nonsmokers. We conducted a randomized, double-blind, placebo-controlled trial comparing the effects of BSH on serially sampled nasal lavage fluid (NLF) cytokines, viral sequence quantity, and Nrf2-dependent enzyme expression in NLF cells and biopsied epithelium. Healthy young adult smokers and nonsmokers ingested BSH or placebo (alfalfa sprout homogenate) for 4 days, designated Days -1, 0, 1, 2. On Day 0 they received standard vaccine dose of live attenuated influenza virus (LAIV) intranasally. Nasal lavage fluids and nasal biopsies were collected serially to assess response to LAIV. In area under curve analyses, post-LAIV IL-6 responses (P = 0.03) and influenza sequences (P = 0.01) were significantly reduced in NLF from BSH-treated smokers, while quinoneoxidoreductasein NLF cells was significantly increased. In nonsmokers, a similar trend for reduction in virus quantity with BSH did not reach statistical significance. In smokers, short term ingestion of broccoli sprout homogenates appears to significantly reduce some virus-induced markers of inflammation, as well as reducing virus quantity. Nutritional antioxidant interventions have promise as a safe, low-cost strategy for reducing influenza risk among smokers and other at risk populations. ClinicalTrials.gov NCT01269723.
Bibliography:Conceived and designed the experiments: TN IJ HaZ HoZ. Performed the experiments: CR TN IJ EGB LR RB MM PM SJ BL. Analyzed the data: TN IJ HaZ HoZ. Contributed reagents/materials/analysis tools: EGB LM RB MM PM SJ BL. Contributed to the writing of the manuscript: TN HaZ HoZ EGB LM RB MM PM SJ BL IJ. None.
Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0098671