B7-H1 expression is associated with poor prognosis in colorectal carcinoma and regulates the proliferation and invasion of HCT116 colorectal cancer cells

• Published in: PloS one Vol. 8; no. 10; p. e76012
• Main Authors: Shi, Sheng-Jia, Wang, Li-Juan, Wang, Guo-Dong, Guo, Zhang-Yan, Wei, Ming, Meng, Yan-Ling, Yang, An-Gang, Wen, Wei-Hong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.10.2013; Public Library of Science (PLoS)
• Subjects: Analysis; Apoptosis; Apoptosis - physiology; B cells; B7-H1 Antigen - metabolism; Biology; Blotting, Western; Cancer; Cancer cells; Cancer therapies; Carcinoma; Cell differentiation; Cell migration; Cell Proliferation; Colon; Colon cancer; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - metabolism; Diagnosis; Diagnostic systems; Differentiation (biology); Electrophoresis, Polyacrylamide Gel; Growth factors; HCT116 Cells; Hospitals; Humans; Immunohistochemistry; Immunology; Laboratories; Ligands; Lymphocytes; Medical diagnosis; Medical prognosis; Medical research; Metastases; Multivariate analysis; Paraffin; Patients; Prognosis; siRNA; Surgery; Urology; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0076012

The investigation concerning the B7-H1 expression in colorectal cancer cells is at an early stage. It is unclear whether B7-H1 expression may have diagnostic or prognostic value in colorectal carcinoma. Additionally, how B7-H1 is associated with the clinical features of colorectal carcinoma is not known. In order to investigate the relationship between B7-H1 and colorectal cancer, we analyzed B7-H1 expression and its effect in clinical specimens and HCT116 cells. Paraffin-embedded specimens from 143 eligible patients were used to investigate the expression of CD274 by immunohistochemistry. We also examined whether B7-H1 itself may be related to cell proliferation, apoptosis, migration and invasion in colon cancer HCT116 cells. Our results show that B7-H1 was highly expressed in colorectal carcinoma and was significantly associated with cell differentiation status and TNM (Tumor Node Metastasis) stage. Patients with positive B7-H1 expression showed a trend of shorter survival time. Using multivariate analysis, we demonstrate that positive B7-H1 expression is an independent predictor of colorectal carcinoma prognosis. Our results indicate that B7-H1 silencing with siRNA inhibits cell proliferation, migration and invasion. Furthermore, cell apoptosis was also increased by B7-H1 inhibition. Positive B7-H1 expression is an independent predictor for colorectal carcinoma prognosis. Moreover, knockdown of B7-H1 can inhibit cell proliferation, migration and invasion.