Synergistic effects of Cu-doped ZnO nanoantibiotic against Gram-positive bacterial strains

• Published in: PloS one Vol. 16; no. 5; p. e0251082
• Main Authors: Khalid, Awais, Ahmad, Pervaiz, Alharthi, Abdulrahman I, Muhammad, Saleh, Khandaker, Mayeen Uddin, Faruque, Mohammad Rashed Iqbal, Din, Israf Ud, Alotaibi, Mshari A, Khan, Abdulhameed
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.05.2021; Public Library of Science (PLoS)
• Subjects: Antibacterial activity; Antibiotics; Antimicrobial agents; Apoptosis; Bacteria; Biocompatibility; Biological properties; Biology and Life Sciences; Biomedical materials; Care and treatment; Chemical properties; Chemistry; Chloride; Copper; Cytotoxicity; Dopants; Drug delivery systems; E coli; Electrical conductivity; Electrical resistivity; Engineering and Technology; Gram-negative bacteria; Gram-positive bacteria; Gram-positive bacterial infections; Health aspects; Humanities; Magnetic properties; Medicine and Health Sciences; Nanoparticles; Optical properties; Pharmaceutical research; Physical properties; Physical Sciences; Physics; Pneumonia; Potash; Potassium; Production processes; Streptococcus infections; Synergistic effect; Toxicity; Zinc; Zinc oxide; Zinc oxides; Pakistan; Saudi Arabia; Selangor Malaysia; Kuala Lumpur Malaysia; Malaysia; Kashmir India; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0251082

A viable hydrothermal technique has been explored for the synthesis of copper doped Zinc oxide nanoparticles (Cu-doped ZnO-NPs) based on the precursor's mixture of Copper-II chloride dihydrate (CuCl2.2H2O), Zinc chloride (ZnCl2), and potassium hydroxide (KOH). X-ray diffraction (XRD) reported the hexagonal wurtzite structure of the synthesized Cu-doped ZnO-NPs. The surface morphology is checked via field emission scanning electron microscopy (FE-SEM), whereas, the elemental compositions of the samples were confirmed by Raman, and X-ray photoelectron spectroscopy (XPS), respectively. The as-obtained ZnO-NPs and Cu-doped ZnO-NPs were then tested for their antibacterial activity against clinical isolates of Gram-positive (Staphylococcus aureus, Streptococcus pyogenes) and Gram-negative (Escherichia coli, Klebsiella pneumonia) bacteria via agar well diffusion method. The zone of inhibition (ZOI) for Cu-doped ZnO-NPs was found to be 24 and 19 mm against S. Aureus and S. pyogenes, and 18 and 11 mm against E. coli and K. pneumoniae, respectively. The synthesized Cu-doped ZnO-NPs can thus be found as a potential nano antibiotic against Gram-positive multi-drug resistant bacterial strains.